Resources
Step-by-step guide (recommended for first time users):
Includes how to use the nitrogen cylinder, switching on the instrument, conducting checks and setting up the experiment.
Relevant papers (detailed guide on CD protocols)
A very comprehensive review by Greenfield. Talks about sample prep, buffer tolerance, list of software that can be used for analysis, protocols etc.
A paper that covers the basic principles of CD, what information you can expect to obtain from a CD spectrum and more on sample prep.
A more recent (2021) review of tools that can be used for CD spectra analysis and case studies. Covers the basics as well.
User manuals
Software manual
Instrument manual
CD video explainers:
Software:
Name Use Website Notes
BestSel CD spectra analysis
Secondary structure prediction http://bestsel.elte.hu/index.php
K2D3 server Secondary structure prediction http://cbdm-01.zdv.uni-mainz.de/~andrade/k2d3/
DichroWeb server Spectra analysis
Secondary structure prediction http://dichroweb.cryst.bbk.ac.uk/html/home.shtml Prior registration required
CDToolX Analysis software Downloadable
The Protein Circular
Dichroism Data Bank
(PCDDB) Online repository for CD spectra https://pcddb.cryst.bbk.ac.uk/