Resources

Step-by-step guide (recommended for first time users):

Includes how to use the nitrogen cylinder, switching on the instrument, conducting checks and setting up the experiment.

Preparing for a new CD run.docx

Relevant papers (detailed guide on CD protocols)

A very comprehensive review by Greenfield. Talks about sample prep, buffer tolerance, list of software that can be used for analysis, protocols etc.

nprot.2006.202.pdf

A paper that covers the basic principles of CD, what information you can expect to obtain from a CD spectrum and more on sample prep.

CD Review 2005.pdf

A more recent (2021) review of tools that can be used for CD spectra analysis and case studies. Covers the basics as well.

d0cs00558d.pdf

User manuals

Software manual

DMS_and_Global_3_User_Manual.pdf

Instrument manual

Chirascan_Series_Manual.pdf

CD video explainers:

Software:

Name Use Website Notes

BestSel CD spectra analysis

Secondary structure prediction http://bestsel.elte.hu/index.php

K2D3 server Secondary structure prediction http://cbdm-01.zdv.uni-mainz.de/~andrade/k2d3/

DichroWeb server Spectra analysis

Secondary structure prediction http://dichroweb.cryst.bbk.ac.uk/html/home.shtml Prior registration required

CDToolX Analysis software Downloadable

The Protein Circular

Dichroism Data Bank

(PCDDB) Online repository for CD spectra https://pcddb.cryst.bbk.ac.uk/