The Ichetucknee River is a spring-fed, pristine river in North Central Florida. The entire 6 miles (9.7 km) of the river average 20 feet (6.1 m) wide, 5 feet (1.5 m) deep and most of the 6 miles lie within the boundaries of the Ichetucknee Springs State Park while the rest is to the south of US Highway 27. Three Rivers Estates Property Owners (TREPO) is the property owner association that manages the area along the private side of the river as it travels and flows into the Santa Fe River.

The water in the river is crystal-clear and is 72 °F (22 °C) year-round. There are nine named springs within the Ichetucknee Springs group with an average total flow of 212 million gallons per day. The group includes: Ichetucknee Springs, Cedar Head Spring, Blue Hole Spring, Roaring Springs, Singing Springs, Boiling Spring, Grassy Hole Springs, Mill Pond Spring, and Coffee Spring. The Ichetucknee is a tributary of the Santa Fe River, which in turn flows into the Suwannee River before emptying into the Gulf of Mexico.

The park restricts launch and end points along the river, permitting a tube/raft excursion of 45 min, 1.5 or 3 hours. A variety of commercial vendors, located outside the entrance to the park, rent tubes and rafts for a modest fee. Park wildlife includes: North American river otter, North American beaver, turtle, ibis, wood stork, egret, great blue heron, cormorant, anhinga, belted kingfisher, limpkin, wild turkey, wood duck, white-tailed deer, raccoon, nine-banded armadillo, wild boar, The fish are primarily bream, bluegill, largemouth bass, alligator gar, mullet, and catfish. West Indian manatees have been seen in the winter months.

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