The project “Flexible PDMS-integrated Plasmonic Paper as Versatile Nanochip for Metal Enhanced Fluorescence Biosensing” (acronym Chip4MEF) aims to design an innovative, versatile hybrid nanochip for highly efficient Metal Enhanced Fluorescence (MEF)–based Point-of-Care (POC) detection enabling the flexibility, miniaturization, portability of the bio(nano)sensors, fast analytical evaluation, reduced costs while achieving a rapid and sensitive MEF detection. Specifically, the originality of this proposal consists in the incorporation of the Whatman paper with pre-immobilized highly efficient anisotropic gold nanobipyramids (AuBPs) or nanorods (AuNRs) in between two polydimethylsiloxane (PDMS) layers with specific configurations, engineering thus an innovative hybrid microfluidic nanochip with enhanced and well-controlled fluorescence sensing capabilities.