The originality and complexity of this project consists in designing a hybrid nanochip which will successfully combine the efficiency of anisotropic AuNPs, simply immobilized into a 3D paper matrix without any complicated surface modification, with the sample-driven advantages of the microfluidic channels. More importantly, by making use of the AuBPs’s and AuNRs’ facile tunability of the narrow longitudinal LSPR response in the Near-Infrared (NIR) region that can easily overlap with the response of the emitter, a novel well-desired NIR-MEF biosensing detection system will be implemented, that -to the best of our knowledge -has not been explored in literature so far. With this aim, the specific objectives are:

(1) Design of well-defined and optimized V-Shaped plasmonic lines via plasmonic calligraphy approach directly on the paper;

(2) Control of the fluorescence enhancement by modulating the separation distance between the fluorophore and calligraphed Au surface;

(3) Fabrication of the Flexible Nanochip based on PDMS-integrated Plasmonic Paper;

(4) Validation and implementation of our optimized PDMS-integrated Plasmonic Paper as a fast, stable and highly sensitive MEF assay.