Chantler labs

Basic science lab: Dr. Chantler has dedicated laboratory areas at the WVU Health Science Center North. This includes 3 rooms (totaling 1100 ft² room for all animal experimentation). This area has dedicated rooms for the study of in-vivo/in-situ animal preparations, isolated microvessel and vascular ring preparations, surgical stations, general laboratory procedures, and administration. Dr. Chantler also has the Laser Speckle Imager, Laser Doppler flow (Moor FLPI-2, MoorVMS-LDF2). A dedicated room within this laboratory is also used for all microscopic/imaging analyses. An additional room (350 ft²) is dedicated for all biochemical and molecular biological assays. Major equipment includes; DMT620M and Living Systems myography packages for pressure and wire myography, ADI PowerLab; Olympus videomicroscope w/fluorescence capabilities, Nikon – Elements software. Zeiss microscope station equipped with digital camera, Physiotemp animal thermal regulating station, Kent Scientific CODA Surgical monitor (for blood pressure), and numerous accessories dedicated for use as an intravital microscope. Relevant equipment include isoflurane anesthesia vaporizer with scavenging system, cigarette smoke machine, Harvard model 683 rodent ventilator, metal warming table with water pump, surgical tools and instruments for rodent surgeries, and access to rodent pulmonary function equipment (i.e. Buxco whole-body plethysmography, FlexiVent by Sceireq). Dr. Chantler maintains a fully functional molecular laboratory with all the necessary equipment for DNA, RNA and protein analysis. Major equipment items include an Eppendorf PCR thermocycler, several gel box systems with power supplies and blot transfer modules, multiple pipettors, an assortment of centrifuges and water baths, stir/hot hotplates, tissue homogenizers, dry-tube bath, Carl Zeiss microscope with ICC3 Zeiss camera (along with analysis software), BIOTEK microplate reader, and storage freezers and refrigerators (i.e., -80C, -20C and 4C).

Human clinical lab: Dr. Chantler's human clinical lab is situated on the ground floor of the HSC south building, and is housed within the Human Performance Lab (https://medicine.hsc.wvu.edu/ep/patient-care/human-performance-lab/). This large CV room is complete with stress test equipment, a parvo medics metabolic cart, phlebotomy supplies and blood sampling equipment. The non-invasive CV unit has numerous SphygmoCor AtCor Medical devices (CvMS and EXCEL) to measure PWV, PWA, and HRV. Portable GE Vivid i and other ultrasound units for the cardiac and vascular assessments. EndoPat 2000 for endothelial function. These equipment allow for a detailed CV assessment. Body composition includes BodPOD and DEXA.