Genetically encoded reporters of actin filament organization in living cells and tissues
Carla Silva Martins, François Iv, Shashi Kumar Suman, Thomas C. Panagiotou, Clara Sidor, María Ruso-López, Camille N. Plancke, Shizue Omi, Maxime Gomes, Alexander Llewellyn, Sourish Reddy Bandi, Laurie Ramond, Federica Arbizzani, Caio Vaz Rimoli, Frank Schnorrer, François Robin, Andrew Wilde, Loïc LeGoff, Jean-Denis Pedelacq, Stéphanie Cabantous, Sergio A. Rincon, Cristel Chandre, Sophie Brasselet, Manos Mavrakis
Featured in the Mechanobiology 2023 collection of JCB!
Featured in the Structural Biology 2023 collection of JCB!
Featured as a Spotlight in the March issue of JCB!
Human septins organize as octamer-based filaments and mediate actin-membrane anchoring in cells
Carla Silva Martins, Cyntia Taveneau, Gerard Castro-Linares, Mikhail Baibakov, Nicolas Buzhinsky, Mar Eroles, Violeta Milanović, Shizue Omi, Jean-Denis Pedelacq, Francois Iv, Léa Bouillard, Alex Llewellyn, Maxime Gomes, Mayssa Belhabib, Mira Kuzmić, Pascal Verdier-Pinard, Stacey Lee, Ali Badache, Sanjay Kumar, Cristel Chandre, Sophie Brasselet, Felix Rico, Olivier Rossier, Gijsje H. Koenderink, Jerome Wenger, Stéphanie Cabantous, Manos Mavrakis
Journal of Cell Biology 222 (3), e202203016 (2023)
The compass to follow: focal adhesion turnover
Manos Mavrakis and Maria Angeles Juanes
Current Opinion in Cell Biology (2023) 80:102152
Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins
Brieuc Chauvin, Koyomi Nakazawa, Alexandre Beber, Aurélie Di Cicco, Bassam Hajj, François Iv, Manos Mavrakis, Gijsje H. Koenderink, João T. Cabral, Michaël Trichet, Stéphanie Mangenot, Aurélie Bertin
Journal of Visualized Experiments 186, e63889 (2022)
Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution
Gerard Castro-Linares, Jeffrey den Haan, Francois Iv, Carla Silva Martins, Aurélie Bertin, Manos Mavrakis, Gijsje H. Koenderink
Journal of Visualized Experiments 184, e63871 (2022)
Septin Filament Compaction Into Rings Requires the Anillin Mid2 and Contractile Ring Constriction
Federica Arbizzani, Manos Mavrakis, Marta Hoya, Juan Carlos Ribas, Sophie Brasselet, Anne Paoletti, Sergio Rincon
Cell Reports 39(3):110722 (2022)
4polar-STORM polarized super-resolution imaging of actin filament organization in cells
Caio Vaz Rimoli, Cesar Augusto Valades Cruz, Valentina Curcio, Manos Mavrakis and Sophie Brasselet
Nature Communications 13, 301 (2022)
Septin-microtubule association requires a MAP-like motif unique to Sept9 isoform 1 embedded into septin octamers
Mira Kuzmić, Gerard Castro Linares, Jindřiška Leischner Fialová, François Iv, Danièle Salaün, Alex Llewellyn, Maxime Gomes, Mayssa Belhabib, Yuxiang Liu, Keisuke Asano, Magda Rodrigues, Daniel Isnardon, Taro Tachibana, Gijsje Koenderink, Ali Badache, Manos Mavrakis, Pascal Verdier-Pinard
Jounal of Cell Science 135(1): jcs.258850 (2022)
Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms
Francois Iv, Carla Silva Martins, Gerard Castro-Linares, Cyntia Taveneau, Pascale Barbier, Pascal Verdier-Pinard, Luc Camoin, Stéphane Audebert, Feng-Ching Tsai, Laurie Ramond, Alex Llewellyn, Mayssa Belhabib, Koyomi Nakazawa, Aurélie Di Cicco, Renaud Vincentelli, Jerome Wenger, Stéphanie Cabantous, Gijsje H. Koenderink, Aurélie Bertin, Manos Mavrakis
Jounal of Cell Science 134 (15): jcs258484 (2021)
Membrane binding controls ordered self-assembly of animal septins
Szuba, A., Bano, F., Iv, F., Mavrakis, M., Richter, R.P., Bertin, A. and Gijsje H. Koenderink
eLife 10:e63349 (2021)
Mitochondrial morphology and activity regulate furrow ingression and contractile ring dynamics in Drosophila cellularization
Chowdhary, S., Madan, S., Tomer, D., Mavrakis, M. and Richa Rikhy
Molecular Biology of the Cell 31(21), 2331-2347 (2020)
The role of APC-mediated actin assembly in microtubule capture and focal adhesion turnover.
Juanes, M.A., Isnardon, D., Badache, A., Brasselet, S., Mavrakis, M. and Bruce Goode
Journal of Cell Biology 218(10), 3415-3435 (2019)
Visualizing septins in early Drosophila embryos.
Mavrakis, M.
Methods in Cell Biology, Volume 136, 199-220 (2016)
Purification of recombinant human and Drosophila septin hexamers for TIRF assays of actin-septin filament assembly.
Mavrakis, M., Tsai, F.-C., and Koenderink, G.H.
Methods in Cell Biology, Volume 136, 183-198 (2016)
Quantitative nanoscale imaging of orientational order in biological filaments by polarized superresolution microscopy.
Cruz, C.V., Shaban, H.A., Kress, A., Bertaux, N., Monneret, S., Mavrakis, M., Savatier, J., Brasselet, S.
PNAS 113(7), E820-8 (2016)
Dynamin regulates metaphase furrow formation and plasma membrane compartmentalization in the syncytial Drosophila embryo.
Rikhy, R., Mavrakis, M., and J. Lippincott-Schwartz
Biology Open 4(3), 301-11 (2015)
Septins promote F-actin ring formation by cross-linking actin filaments into curved bundles.
Mavrakis, M., Azou-Gros, Y., Tsai, F.-C., Alvarado, J., Bertin, A., Iv, F., Kress, A., Brasselet, S., Koenderink, G.H. and T. Lecuit
Nature Cell Biology 16 (4), 322-334 (2014)
(rated as "exceptional" by F1000Prime; featured in Curr. Biol. (2014) 24(11), R526)
Lighting up developmental mechanisms: how fluorescence imaging opened a new era.
Mavrakis, M., Pourquié, O., and T. Lecuit
Development 137, 373-387 (2010)
Cells within a cell: insights into cellular architecture and polarization from the organization of the early fly embryo.
Mavrakis, M., Rikhy, R., and J. Lippincott-Schwartz
Communicative & Integrative Biology 2 (4), 313-4 (2009)
Plasma Membrane Polarity and Compartmentalization are Established prior to Cellularization in the Fly Embryo.
Mavrakis, M., Rikhy, R., and J. Lippincott-Schwartz
Developmental Cell 16, 93-104 (2009)
(cited as a "must read" by F1000)
Fluorescence Imaging Techniques for Studying Drosophila Development.
Mavrakis, M., Rikhy, R., Lilly, M., and Lippincott-Schwartz, J.
Current Protocols in Cell Biology, Chapter 4: Unit 4.18 (2008)
The secretory membrane system in the Drosophila syncytial blastoderm embryo exists as functionally compartmentalized units around individual nuclei.
Mavrakis, M.*, Frescas, D.*, Lorenz, H., DeLotto, R., and J. Lippincott-Schwartz
Journal of Cell Biology 173, 219-230 (* equal contribution) (2006)
previous work on autophagy and viral protein complexes
Protein kinase A effects of an expressed PRKAR1A mutation associated with aggressive tumors.
Meoli, E., Bossis, I., Cazabat, L., Mavrakis, M., Horvath, A., Shiferaw, M.L., Fumey, G., Perlemoine, K., Muchow, M., Robinson-White, A., Weinberg, F., Nesterova, M., Patronas, Y., Groussin, L., Bertherat, J., and Stratakis, C.A.
Cancer Research 68, 3133-3141 (2008)
Homeostatic Mechanisms for Iron Storage Revealed by Genetic Manipulations and Live Imaging of Drosophila Ferritin.
Missirlis, F., Kosmidis, S., Brody, T., Mavrakis, M., Holmberg, S., Odenwald, W.F., Skoulakis, E.M.C., and Rouault, T.A.
Genetics 177, 89-100 (2007)
mTOR kinase and the regulatory subunit of protein kinase A (PRKAR1A) spatially and functionally interact during autophagosome maturation.
Mavrakis, M., Lippincott-Schwartz, J., Stratakis, C.A., and Bossis, I.
Autophagy 3, 151-153 (2007)
Depletion of type IA regulatory subunit (RIa) of protein kinase A (PKA) in mammalian cells and tissues activates mTOR and causes autophagic deficiency.
Mavrakis, M., Lippincott-Schwartz, J., Stratakis, C.A., and Bossis, I.
Human Molecular Genetics 15, 2962-2971 (2006)
Rabies virus chaperone: Identification of the phosphoprotein peptide that keeps nucleoprotein soluble and free from non-specific RNA.
Mavrakis, M., Méhouas, S., Réal, E., Iseni, F., Blondel, D., Tordo, N., and Ruigrok, R.W.
Virology 349, 422-429 (2006)
Rab9 GTPase is required for replication of human immunodeficiency virus type 1, filoviruses, and measles virus.
Murray, J.L., Mavrakis, M., McDonald, N.J., Yilla, M., Sheng, J., Bellini, W.J., Zhao, L., Le Doux, J.M., Shaw, M.W., Luo, C., Lippincott-Schwartz, J., Sanchez, A., Rubin, D.H., and T.W. Hodge
Journal of Virology 79, 11742-11751 (2005)
Structure and function of the C-terminal domain of the polymerase cofactor of rabies virus.
Mavrakis, M., McCarthy, AA., Roche, S., Blondel, D., and Ruigrok, R.W.
Journal of Molecular Biology 343, 819-831 (2004)
The 12Å structure of trypsin-treated measles virus N-RNA.
Schoehn, G., Mavrakis, M., Albertini, A., Wade, R., Hoenger, A., and Ruigrok, R.W.
Journal of Molecular Biology 339, 301-312 (2004)
Isolation and characterisation of the rabies virus No-P complex produced in insect cells. Mavrakis, M., Iseni, F., Mazza, C., Schoehn, G., Ebel, C., Gentzel, M., Franz, T., and Ruigrok, R.W.
Virology 305, 406-414 (2003)
Morphology of Marburg virus NP-RNA.
Mavrakis, M., Kolesnikova, L., Schoehn, G., Becker, S., and Ruigrok, R.W.
Virology 296, 300-307 (2002)
(cover image of the journal)
Structure of recombinant rabies virus nucleoprotein-RNA complex and identification of the phosphoprotein binding site.
Schoehn, G., Iseni, F., Mavrakis, M., Blondel, D., and Ruigrok, R.W.
Journal of Virology 75, 490-498 (2001)
(cover image of the journal)