Research

Can Membrane tension and fluctuations affect membrane fusion during myogenesis?

Now online: Chakraborty M, Sivan A, Biswas A and Sinha B (2022) Early tension regulation coupled to surface myomerger is necessary for the primary fusion of C2C12 myoblasts. Front. Physiol. 13:976715. doi: 10.3389/fphys.2022.976715

Collective cell migration : is the role of membrane tension same as single cell motility? are there cell -to- cell differences?

While finding about that, we found that collective cell migration in fish keratocytes has an unique cell-substrate adhesion profile. Some cells following the leader cells actually "take a ride" - they give away their adhesion with the substrate and in turn help the leader be more "directional". Read more here in the Bio Open paper:

Effect of heterogeneous substrate adhesivity of follower cells on speed and tension profile of leader cells in primary keratocyte collective cell migration

Mapping membrane height fluctuations; fluctuation-maps to membrane mechanical parameters?

Biswas, Arikta; Alex, Amal and Sinha, Bidisha. 2017. "Mapping cell membrane fluctuations reveals their active regulation and transient heterogeneities." Biophysical Journal, 113, 1768-1781

http://dx.doi.org/10.1016/j.bpj.2017.08.041

How does the thin (~500nm) actin cortex respond to mechanical perturbations? We study role cell spread area in controlling cortex thickness and find Traction forces to be very important. Rinku's Preprint: https://www.biorxiv.org/content/early/2017/10/18/205138

Manuscript in press @ BBA Molecular Cell Research

available online : https://doi.org/10.1016/j.bbamcr.2019.07.011

>More coming soon. We will talk about how membrane-cytoskeleton linkages matter!

Excess membrane just after de-adhesion - how do fluctuations "flow"? How does the tension profile evolve? Is the set point of tension altered when a cell is restricted to smaller spread area (hence thicker actin cortex)?

>Soon.. we will get back with updates

Are there special regions on the plasma membrane where membrane trafficking is more prone to happen?

> Tithi thinks so. But let's be super sure and we get back with something for you to read.

Is the Golgi reorientation during wound healing a way to alter 3D distribution of exocytotic events?