Regulation of cellular metabolism is a fundamental trait of all living organisms. For long considered a housekeeping process mainly aimed to convert nutrients into energy and biomass, it is becoming increasingly evident that metabolism is actively involved in the control of many physiological processes. The overarching goal of the Sebastián laboratory is to understand the role of metabolism as a regulator of stem cell fate, tissue homeostasis, and tumorigenesis, and the functional interplay between metabolic reprogramming and other genetic and epigenetic programs involved in these processes.  To do this we employ stem cell cultures, 3D-organoids systems, genetically modified mouse models, tumor samples, patient-derived organoids and genetically encoded metabolic reporters.