Publications

P. R. Oliveira-Pinto, J. Oliveira-Fernandes, D. Gramaje, C. Santos. 2025. Metabarcoding profiling reveals microbiome structure and predicts functional shifts in grapevine challenged by Phyllostica ampelicida. Microbial Ecology 88:120

D. Labarga, A. Mairata, M. Puelles, D. Gramaje, A. Pou. 2025. Ozonated irrigation in vineyards: limited effects on soil bacterial and fungal microbiota. Pest Management Science (in press)

E. Abarquero, M. P. Martínez-Diz, A. Díaz-Fernández, D. Gramaje, E. Díaz-Losada. 2025. Cultivar-specific effects of physical and biological treatments on grapevine trunk disease control and plant vigour. Phytopathologia Mediterranea 64:513-526

K. Stuskova, T. Kiss, Z. Bytesnikova. L. Richtera, D. Gramaje, A. Eichmeiers. 2025. Silver-selenium nanoparticles and selcected chemical compounds significantly inhibit grapevine trunk diseases pathogens. Pest Management Science 81:7985-7994

F. Azevedo.Nogueira, A. Gaspar, J. Lopes, C. Rego, D. Gramaje, H. Gonçalves, P. Martins-Lopez. 2025. Regional influence and cultivar predisposition affect the prevalence of grapevine fungal pathogens in Portuguese vineyards. Pest Management Science 81:8383-8394

C. Leal, M.J. Carbone, A. Eichmeier, T. Kiss, D. Tekielska, L. Rivacoba, R. Bujanda, D. Gramaje. 2025. Shifting fungal networks: how Dactylonectria macrodidyma shapes grapevine mycobiome in diverse soils. Phytobiome Journal (in press)

F. Azevedo-Nogueira, A. Gaspar, K. Stuskova, A. Margarida-Fortes, A. Eichmeier, C. Rego, D. Gramaje, P. Martins-Lopez. 2025. Microbiome composition in grapevine trunk diseases symptomatic plants is modulated by genotype and region. Scientific Reports (in press)

C. Leal, L. Hech, I. Saber, D. Aggad, N. Richet, D. Gramaje, J. Armengol, F. Fontaine, P. Trotel-Aziz. 2025. RNA-seq reveals differential responses in Vitis vinifera cultivars protected by distinct biocontrol agents agasint Neofusicoccum parvum Bt67. Phytopathology 115:1447-1464

J.F. Aguiar, I.M. Liberal, M.L. Muñoz, E. Cantos-Villar, B. Puertas, A. Archidona-Yuste, R. Casimiro-Soriguer, M. Coletta, A. D'Alessandro, A. González-Robes, D. Gramaje, D. Renard, M. Ray, A. La Terza, R. Tarifa, C. Vacher, C. Veen, R. Ochoa-Hueso. 2025. Development of whole-soil microbial inoculants based on solid-phase fermentation for the regeneration of the functionaing of vineyard soils. Rhizosphere (in press)

K. Stuskova, A. Vavrinik, E. Hakalova, J. Cechova, D. Gramaje, A. Eichmeier. 2025. Arbuscular mycorrhizal fungi strongly influence the endorhizosphere of grapevine rootstock with soil type as a key factor. Mycorrhiza 35, 17

D. Labarga, A. Mairata, M. Puelles, J. Tronchoni, A. Eichmeier, M. de Toro, D. Gramaje, A. Pou. 2025. Vineyard mycobiota shows a local and long-term response to the organic mulches application. Agriculture, Ecosystems and Environment 382:109506

A. Villa-Llop, S. Crespo-Martínez, D. Marín, N. Torres, S. Jane-Cookson, G. Loupit, P.O. Bonhomme, D. Prodhomme, D. Gramaje, R. Bujanda, J. Eraso, L.G. Santesteban. 2025. Evaluation of the influence of rootstock cane characteristics on grafting success rate. OENO One 59:1

C. Leal, R. Bujanda, B. López-Manzanares, S. Ojeda, M. Berbegal, A. Villa-Llop, L.G. Santesteban, J. Palacios, D. Gramaje. 2024. Evaluating treatments for the protection of grapevine pruning wounds from natural infection by trunk disease fungi. Plant Disease 108:3052-3062.

C. Leal, R. Bujanda, M.J. Carbone, T. Kiss, A. Eichmeier, D. Gramaje, M.M. Maldonado-González. 2024. Drought influences the fungal community structure, diversity, and functionality inhabiting the grapevine xylem and enhances the abundance of Phaeomoniella chlamydospora. Phytobiomes Journal https://doi.org/10.1094/PBIOMES-02-24-0027-R

C. Leal, A. Eichmeier, K. Stuskova, J. Armengol, R. Bujanda, F. Fontaine, P. Trotel-Aziz, D. Gramaje. 2024. Biocontrol agents establishment and their impact on rhizosphere microbiome and induced grapevine defenses is highly soil-dependent. Phytobiomes Journal https://apsjournals.apsnet.org/doi/10.1094/PBIOMES-08-23-0077-R

C. Leal, D. Gramaje. 2024. Management strategies for reducing pesticide use against diseases caused by fungi and oomycetes in grapevine. Advances in Botanical Research 10:197-253.

L. Mostert, M. Whipp, A. Woodgyer, R.C. Summerbell, D. Gramaje, C.F.J. Spies. 2024. Phaeoacremonium tuscanicum and Phaeoacremonium indicum sp. nov. associated with subcutaneous phaeohyphomycosis. Mycology https://doi.org/10.1080/21501203.2024.2312917

C. Leal, P. Trotel-Aziz, D. Gramaje, J. Armengol, F. Fontaine. 2024. Exploring some factors conditioning the expression of Botryosphaeria dieback in grapevine for an integrated management of the disease. Phytopathology 114:21-34.

Visagie, C. M., Magistà, D., Ferrara, M., Balocchi, F., Duong, T. A., Eichmeier, A., Gramaje, D., Aylward, J., Baker, S. E., Barnes, I., Calhoun, S., De Angelis, M., Frisvad, J. C., Hakalova, E., Hayes, R. D., Houbraken, J., Grigoriev, I. V., LaButti, K., Leal, C., Lipzen, A., Ng, V., Pangilinan, J., Pecenka, J., Perrone, G., Piso, A., Savage, E., Spetik, M., Wingfield, M. J., Zhang, Y., Wingfield, B. D.. 2023. The re-identification of Penicillium genomes available in NCBI and draft genomes for Penicillium species from dry cured meat, Penicillium biforme, P. brevicompactum, P. solitum, and P. cvjetkovicii, Pewenomyces kutranfy, Pew. lalenivora, Pew. tapulicola, Pew. kalosus, Teratosphaeria carnegiei, and Trichoderma atroviride SC1. IMA Fungus 14:21.

P.W. Crous, E.R. Osieck, R.G. Shivas, S.L. Bishop-Hurley, F. Esteve-Raventós et al. 2023. Fungal Planet description sheets: 1478-1549. Persoonia 50:158-310.

G.Bortolami, N. Ferrer, K. Baumgartner, S. Delzon, D. Gramaje, L.J. Lamarque, G. Romanazzi, G.A. Gambetta, C. Delmas. 2023. Esca grapevine disease involves leaf hydraulic failure and represents a unique premature senescense process. Tree Physiology 43:441-451.

C. Leal, D. Gramaje, F. Fontaine, N. Richet, P. Trotel-Aziz, J. Armengol. 2023. Evaluation of Bacillus subtilis PTA-271 and Trichoderma atroviride SC1 to control Botryosphaeria dieback and black-foot pathogens in grapevine propagation material. Pest Management Science 79:1674-1683.

V. Guarnaccia, C. Kraus, E. Markakis, A. Alves, J. Armengol, A. Eichmeier, S. Compant, D. Gramaje. 2022. Fungal trunk diseases of fruit trees in Europe: pathogens, spread and future directions. Phytopathologia Mediterranea 61:563-599.

F. Azevedo-Nogueira, C. Rego, H.M.R. Gonçalves, A.M. Fortes, D. Gramaje, P. Martins-Lopes. 2022. The road to molecular identification and detection of fungal grapevine trunk diseases. Frontiers in Plant Science 13:960289

A. Eichmeier, E. Díaz-Losada, E. Hakalova, J. Pecenka, K. Stuskova, S. Ojeda, D. Gramaje. 2022. Draft genome sequence of Phyllosticta ampelicida, the cause of grapevine black rot. Phytopathologia Mediterranea 6:39-42

Q. Chen, M. Bakhshi, Y. Balci, K.D. Broders, R. Cheewangkoon, S.F. Chen, X.L. Fan, D. Gramaje, F. Halleen, M. Horta Jung, N. Jiang, T. Jung, T. Májek, S. Marincowitz, I. Milenković, L. Mostert, C. Nakashima, I. Nurul Faziha, M. Pan, M. Raza, B. Scanu, C.F.J. Spies, L. Suhaizan, H.  Suzuki, C.M. Tian, M. Tomšovský, J.R. Úrbez-Torres, W. Wang, B.D. Wingfield, M.J. Wingfield, Q. Yang, X. Yang, R. Zare, P. Zhao, J.Z. Groenewald, L. Cai, P.W. Crous. 2022. Genera of Phytopathogenic Fungi: GOPHY 4. Studies in Mycology 101:417-564

P.W. Crous, M. Sandoval-Denis, M.M. Costa, J.Z. Groenewald et al. (D. Gramaje posición 24 de 59). 2022. Fusarium and allied fusarioid taxa (FUSA). 1. Fungal Systematics and Evolution 9:161-199

D. Gramaje, A. Eichmeier, M. Spetik, M.J. Carbone, R. Bujanda, J. Vallance, P. Rey. 2022. Exploring the temporal dynamics of the fungal microbiome in rootstocks, the lesser-known half of the grapevine crop. Journal of Fungi 8:421

M.V. Longone, G. Escoriaza, M. Paolinelli, D. Gramaje. 2022. First report of Dacylonectria alcacerensis, Dactylonectria macrodidyma and Ilyonectria liriodendri associated with black-foot disease of grapevine in Argentina. Plant Disease in press

S. Vezzulli, D. Gramaje, J. Tello, G. Gambino, A. Schwandner, P. Barba, P. Bettinelli, C. Pirrello, E. Angelini, G.F. Anfora, V. Mazzoni, A. Pozzebon, J.E. Palomares-Rius, M.P. Martínez-Diz, S.L. Toffolatti, G. De Lorenzis, I. Perrone, E. D’Incà, S. Zenoni, J. Wilmink, T. Lacombe, M. Crespan, M.A. Walker, L. Bavaresco, M. de la Fuente, A. Fennel, G.B. Tornielli, A. Forneck, J. Ibañez, L. Hausmann, I.B. Reisch. 2022. Designing for Biotic Stress Resistant Grapevine. In: Kole C (ed) Genomic Designing for Biotic Stress Resistant Fruit Crops. Springer Nature ISBN: 978-3-030-91801-9

M. Spetik, A. Berraf-Tebbal, D. Gramaje, A. Eddine-Mahamedi, K. Stusková, J. Burgova, A. Eichmeier. 2022. Paecilomyces clematidis (Eurotiales, Thermoascaceae): a new species from Clematis root. Phytotaxa 559(3):238-246

C.Leal, N. Richet, J.-F. Guise, D. Gramaje, J. Armengol, F. Fontaine, P. Trotel-Aziz. 2021. Cultivar contributes to the beneficial effects of Bacillus subtilis PTA-271 and Trichoderma atroviride SC1 to protect grapevine against Neofusicoccum parvum. Frontiers in Microbiology 12:726132

M.P. Martínez-Diz, E. Díaz-Losada, M. Andrés-Sodupe, R. Bujanda, M.M. Maldonado-González, S. Ojeda, A. Yacoub, P. Rey, D. Gramaje. 2021. Field evaluation of biocontrol agents against black-foot and Petri diseases of grapevine. Pest Management Science 77:697-708.

C.K. Ramsing, D. Gramaje, S. Mocholí, J. Agustí, F. Cabello Sáenz de Santa María, J. Armengol, M. Berbegal. 2021. Relationship between the xylem anatomy of grapevine rootstocks and their susceptibility to Phaeoacremonium minimum and Phaeomoniella chlamydospora. Frontiers in Plant Science 12:726461

M.J. Carbone, S. Alaniz, P. Mondino, M. Gelabert, A. Eichmeier, D. Tekielska, R. Bujanda, D. Gramaje. 2021. Drought influences fungal community dynamics in the grapevine rhizosphere and root microbiome. Journal of Fungi 7:686.

D. Marín, J. Armengol, P. Carbonell-Bejerano, J.M. Escalona, D. Gramaje, E. Hernández-Montes, D.S. Intrigliolo, J.M. Martínez-Zapater, H. Medrano, J.M. Mirás-Avalos, J.E. Palomares-Rius, P. Romero-Azorín, R. Savé, L.G. Santesteban, F. de Herralde. 2021. Challenges of viticulture adaptationto global change: tackling the issue from the roots. Australian Journal of Grape and Wine Research 27:8-25.

M.P. Martínez-Diz, E. Díaz-Losada, A. Díaz-Fernández, Y. Bouzas-Cid, D. Gramaje. 2021. Protection of grapevine pruning wounds against Phaeomoniella chlamydospora and Diplodia seriata by commercial biological and chemical methods. Crop Protection 143:105465

J.L. Chacón-Vozmediano, D. Gramaje, M. León, J. Armengol, J. Moral, P.M. Izquierdo, J. Martínez-Gascueña. 2021. Cultivar susceptibility to natural infections caused by fungal grapevine trunk pathogens in La Mancha designation of origin (Spain). Plants 10

W. Aigoun-Mouhous, A. E. Mahamedi, M. León, C. Chaouia, A. Zitouni, K. Barankova, A. Eichmeier, J. Armengol, D. Gramaje and A. Berraf-Tebbal. 2021. Cadophora sabaouae sp. nov. and Phaeoacremonium species associated with Petri disease on grapevine propagation material and young grapevines in Algeria. Plant Disease

L. F. Arias-Giraldo, G. Guzmán, M. Montes-Borrego, D. Gramaje, J.A. Gómez and B.B. Landa. 2021. Going Beyond Soil Conservation with the Use of Cover Crops in Mediterranean Sloping Olive Orchards. Agronomy 11,1387.

P. W. Crous, D. A. Cowan, G. Maggs-Kölling et al. 2021. Fungal Planet description sheets: 1182-1283. Persoonia 46:313-528.

P. W. Crous, L. Lombard, M. Sandoval-Denis et al. 2021. Fusarium: more than a node or a foot-shaped basal cell. Studies in Mycology 98:100116.

M.P. Martínez-Diz, A. Eichmeier, M. Spetik, R. Bujanda, A. Díaz-Fernández, E. Díaz-Losada, D. Gramaje. 2021. Grapevine pruning time affects natural wound colonization by wood-invading fungi. Fungal Ecology 48:100994

D. Gramaje, C. Berlanas, M.P. Martínez-Diz, E. Diaz-Losada, L. Antonielli, S. Beier, M. Gorfer, M. Schmoll, S. Compant. 2020. Comparative Genomic Analysis of Dactylonectria torresensis Strains from Grapevine, Soil and Weed Highlights Potential Mechanisms in Pathogenicity and Endophytic Lifestyle. Journal of Fungi 6, 255.

M.P. Martínez-Diz, M. Andrés-Sodupe, M. Berbegal, R. Bujanda, E. Díaz-Losada, D. Gramaje. 2020. Droplet digital PCR technology for detection of Ilyonectria liriodendri from grapevine environmental samples. Plant Disease 104:1144-1150.

C. Berlanas, S. Ojeda, B. López-Manzanares, M. Andrés-Sodupe, R. Bujanda, M.P. Martínez-Diz, E. Díaz-Losada, D. Gramaje. 2020. Occurrence and diversity of black-foot disease fungi in symptomless grapevine nursery stock in Spain. Plant Disease 104:94-104.

A. Eichmeier, J. Pecenka, M. Spetik, T. Necas, I. Ondrasek, J. Armengol, M. León, C. Berlanas, D. Gramaje. 2020. Fungal trunk pathogens associated with Juglans regia in the Czech Republic. Plant Disease 104:761-771

E. González-Domínguez, C. Berlanas, D. Gramaje, J. Armengol, V. Rossi, M. Berbegal. 2020. Temporal dispersal patterns of Phaeomoniella chlamydospora, causal agent of Petri disease and esca, in vineyards. Phytopathology 110:1216-1225.

M.M. Maldonado-González, M.P. Martínez-Diz, M. Andrés-Sodupe, R. Bujanda, E. Diaz-Losada, D. Gramaje. 2020. Quantification of Cadophora luteo-olivacea from grapevine nursery stock and vineyard soil using droplet digital PCR. Plant Disease 104:2269-2274.

J.L. Chacón, D. Gramaje, P.M. Izquierdo, J. Martínez, A. Mena. 2020. Evaluation of six red grapevine cultivars inoculated with Neofusicoccum parvum. European Journal of Plant Pathology 158:811-815.

N. Esparham, H. Mohammadi, D. Gramaje. 2020. A survey of trunk disease pathogens within citrus trees in Iran. Plants 9(6):754.

M. León, M. Berbegal, J.M. Rodríguez-Reina, G. Elena, P. Abad-Campos, A. Ramón-Albalat, D. Olmo, A. Vicent, J. Luque, X. Miarnau, C. Agustí-Brisach, A. Trapero, N. Capote, F.T. Arroyo, M. Avilés, D. Gramaje, M. Andrés-Sodupe, J. Armengol. 2020. Identification and characterization of Diaporthe spp. associated with twig cankers and shoot blight of almonds in Spain. Agronomy 10:1062.

M.P. Martínez-Diz, M. Andrés-Sodupe, R. Bujanda, E. Díaz-Losada, A. Eichmeier, D. Gramaje. 2019. Soil-plant compartments affect fungal microbiome diversity and composition in grapevine. Fungal Ecology 41:234-244.

C. Berlanas, M. Berbegal, G. Elena, M. Laidani, J.F. Cibriain, A. Sagües-Sarasa, D. Gramaje. 2019. The fungal and bacterial rhizosphere microbiome associated with grapevine rootstock genotypes in mature and young vineyards. Frontiers in Microbiology 10:1142.

Y. Marin-Felix, M. Hernández-Restrepo, M.J. Wingfield, A. Akulov, A.J. Camegies, R. Cheewangkoon, D. Gramaje et al. 2019. Genera of Phytopathogenic Fungi: GOPHY 2. Studies in Mycology 92: 47-133.

M. P. Martínez-Diz, E. Díaz-Losada, E. Barajas, D. Ruano-Rosa, M. Andrés-Sodupe, D. Gramaje. 2019. Screening of Spanish Vitis vinifera germplasm for resistance to Phaeomoniella chlamydospora. Scientia Horticulturae 246: 104-109.

E. González-Dominguez, G. Fedele, T. Caffi, L. Deliere, P. Sauris, D. Gramaje, J.L. Ramos-Saéz de Ojer, E. Díaz-Losada, A. Díez-Navajas, P. Bengoa, V. Rossi. 2019. A network meta-analysis provides new insight into fungicide scheduling for the control of Botrytis cinerea in vineyards. Pest Management Science 75:324-332.

S. Panahandeh, H. Mohammadi, D. Gramaje. 2019. Trunk disease fungi associated with Syzygium cumini in Iran. Plant Disease 103:711-720.

P.W. Crous, A. Carnegie, M.J. Wingfield, R. Sharma, G. Muhini, M. Noordeloos, A. Santini et al. 2019. Fungal Planet Description Sheets 868-950. Persoonia 42:291-473.

A. Eichmeier, T. Kiss, E. Penazova, J. Pecenka, A. Berraf-Tebbal, M. Baranek, R. Pokluda, J. Cechova, D. Gramaje, D. Grzebelus. 2019. MicroRNAs in Vitis vinifera cv. Chardonnay are differentially expressed in response to Diaporthe species. Genes 10:905.

B. Mora-Sala, D. Gramaje, P. Abad-Campos, M. Berbegal. 2019. Diversity of Phytophthora species associated with Quercus ilex L. in three Spanish regions evaluated by NGS. Forests 10:979.

A. Eichmeier, J. Pecenka, E. Penazova, M. Baránek, S. Català-García, M. León, J. Armengol, D. Gramaje. 2018. High-throughput amplicon sequencing-based analysis of active fungal communities inhabiting grapevine after hot-water treatments reveals unexpectedly high fungal diversity. Fungal Ecology 36: 26-38

H. Waite, J. Armengol, R. Billones-Baaijens, D. Gramaje, F. Halleen, S. Di Marco, R. Smart. 2018. A protocol for the management of grapevine rootstock mother vines to reduce latent infections by grapevine trunk pathogens in cuttins. Phytopathologia Mediterranea 57: 384-398

C. Berlanas, M. Andrés-Sodupe, B. López-Manzanares, M.M. Maldonado-González, D. Gramaje. 2018. Effect of white mustard cover crop residue, soil chemical fumigation and Trichoderma spp. root treatment on black-foot disease control in grapevine. Pest Management Science 74: 2864-2873.

D. Gramaje, J.R. Úrbez-Torres, M. Sosnowski. 2018. Managing Grapevine Trunk Diseases with respect to etiology and epidemiology: Current Strategies and Future Prospects. Plant Disease 102: 12-39.

V. Guarnaccia, J.Z. Groenewald, J. Woodhall, J. Armengol, T. Cinelli, A. Eichmeir, D. Ezra, F. Fontaine, D. Gramaje, A. Gutierrez-Aguirregabiria, J. Kaliterna, L. Kiss, P. Larignon, J. Luque, L. Mugnai, V. Naor, R. Raposo, E. Sándor, K.Z. Váczy, P.W. Crous. 2018. Diaporthe diversity and pathogenicity revealed from a broad survey of grapevine disease in Europe. Persoonia 40: 135-153.

M.P. Martínez-Diz, E. Díaz-Losada, J. Armengol, M. León, C. Berlanas, M. Andrés-Sodupe, D. Gramaje. 2018. First report of Ilyonectria robusta causing black foot disease of grapevine in Spain. Plant Disease 102: 2381.

C. Berlanas, B. López-Manzanares, D. Gramaje. 2017. Estimation of viable propagules of black-foot disease pathogens in grapevine cultivated soils and their relation to production systems and soil properties. Plant and Soil 417:467-479.

D. Olmo, D Gramaje, J. Armengol. 2017. Evaluation of fungicides to protect pruning wounds from Botryosphaeriaceae species infections on almond trees. Phytopathologia  Mediterranea  56:77-86.

M. Araújo da Silva, K. Câmara Correia, M.A. Guimarâes Barbosa, M.P. Saraiva Câmara, D. Gramaje, S.J. Michereff. 2017. Characterization of Phaeoacremonium isolates associated with Petri disease of table grape in Northeastern Brazil, with description of Phaeoacremonium nordesticola sp. nov. European Journal of Plant Pathology 149:695-709.

D. Gramaje, K. Baumgartner, F. Halleen, L. Mostert, J.R. Úrbez-Torres, M. Sosnowski, J. Armengol. 2016. Fungal trunk diseases: a problem beyond grapevines? Plant Pathology 65:356-357.

D. Olmo, J. Armengol, M. León, D. Gramaje. 2016. Characterization and pathogenicity of Botryosphaeriaceae species isolated from almond trees on the island of Mallorca (Spain). Plant Disease 100: 2483-2491.

M. Marín-Terrazas, J.L. Ramos-Sáez de Ojer, B. López-Manzanares, D. Gramaje. 2016. First report of Phaeoacremonium minimum associated with wood decay of almond trees in Spain. Plant Disease 100: 1244.

D. Gramaje, L. Mostert, J.Z. Groenewald, P.W. Crous. 2015. Phaeoacremonium: from esca disease to phaeohyphomycosis . Fungal Biology 119: 759-783.

D. Gramaje, S. Di Marco. 2015. Identifying practices likely to have impacts on grapevine trunk disease infections: a European nursery survey. Phytopathologia Mediterranea 54: 313-324.

D Olmo, M León, J Armengol, D Gramaje. 2015. Pathogenicity testing of lesser-known fungal trunk pathogens associated with wood decay of almond trees. European Journal of Plant Pathology 143: 607-611.