Objectives and work packages

BioCircus is divided in 4 scientific objectives that will be accomplished through 3 Work Packages:

• Objective 1. Build new biosensor circuits that efficiently detect a range of extracellular concentrations (1 µM to 1 mM) of naringenin and other relevant intermediates of the pathway in E. coli [WP1].

• Objective 2. Build dynamic regulation circuits that control naringenin production in E. coli in response to the accumulation of intracellular naringenin and intermediates trying at the same time to maximize the yield. Test and compare the robustness (i.e., effective, and autonomous adaptation to changing concentrations of these metabolites) and the production profiles to obtain a dynamic production behavior [WP2].

• Objective 3. Test the developed circuits and models in a pre-industrial setting during the industrial placement. Adjust the methods and models to improve the robustness of the developed circuits in a real-world bioproduction scenario [WP2].

• Objective 4. Develop integrated data models using the gathered sensing, bioproduction and robustness data to improve the biosensors and the dynamic control circuits performance over several iterations of the cycle [WP3].