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DNA Assays
DNA Assays
Important Points
Important Points
1. Make sure the buffer composition matches the specifications of the assay (applies to all assays).
2. Equilibrate the reagents at room temperature for 30 min (applies to all assays).
3. Vortex the vials and spin down before use (applies to all assays).
4. Extraction of the samples uses a wide number of chemicals which can affect the results on the Bioanalyzer. It is best practice to run the samples in TE buffer. For HS DNA, do not run samples in water.
5. Standard DNA assays chips are interchangable. For the highsensitivity DNA assay, the HS DNA Chip is required.
6. For HS DNA assay, do not use RNaseZap for cleaning the pinset in between runs. (Residual RNaseZap or SDS on the pins will result in white bands on the gel-like image)
RNA Assays
RNA Assays
Important Points
Important Points
1. Wear gloves when handling RNA samples and reagents.
2. Use RNase-free microfuge tubes, tips and water.
3. Heat-denature RNA samples and Ladder at 70°C for 2 min and keep them on ice to reduce formation of secondary structure. This is especially important for the ladder as this is used for quantification.
4. For RNA Pico and Small RNA assays, do not use RNaseZap for cleaning in between runs. Residual RNaseZap will show up as an overwhelming strong peak in the electropherogram.
5. Small RNA gel is very viscous, therefore when preparing the gel /dye mix for this assay, it is important that add the dye first and then the gel is added on top.
6. Every assay (RNA Nano, RNA Pico, Small RNA) uses its own chip.
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