Papers

2025

2024

■DNA折り紙系ナノデバイスを用いた臓器特異的遺伝子発現制御 

Yuxiang Liu、Ruixuan Wang、Qimingxing Chen、Yan Chang、Qi Chen、Kodai Fukumoto、Bingxun Wang、 Jianchen Yu、Changfeng Luo、Jiayuan Ma、Xiaoxia Chen、Yuko Murayama、Kenichi Umeda、Noriyuki Kodera、 Yoshie Harada、Shun-ichi Sekine、Jianfeng Li、Hisashi Tadakuma

Nano Letters 24(27) 8410-8417 2024年6月26日  査読有り

2023

2022

■Heat-hypersensitive mutants of ryanodine receptor type 1 revealed by microscopic heating

Kotaro Oyama†*, Vadim Zeeb†*, Toshiko Yamazawa†, Nagomi Kurebayashi, Fuyu Kobirumaki-Shimozawa, Takashi Murayama, Hideto Oyamada, Satoru Noguchi, Takayoshi Inoue, Yukiko U. Inoue, Ichizo Nishino, Yoshie Harada, Norio Fukuda*, Shin’ichi Ishiwata, Madoka Suzuki*

Proceedings of the National Academy of Sciences of the United States of America, 119(32), e2201286119 (2022). 

■Modulation of local cellular activities using a photothermal dye-based subcellular-sized heat spot

Ferdinandus, Madoka Suzuki, Cong Quang Vu, Yoshie Harada, Satya Ranjan Sarker, Shin’ichi Ishiwata, Tetsuya Kitaguchi, and Satoshi Arai

ACS Nano , 16, 6, 9004–9018（2022). 

■Opto-thermal technologies for microscopic analysis of cellular temperature-sensing systems

Kotaro Oyama†*, Shuya Ishii†, and Madoka Suzuki*

Biophysical Reviews, 14, 41-5 (2022). 

■Nanaomycin E inhibits NLRP3 inflammasome activation by preventing mitochondrial dysfunction

Yudai Matsui, Naoki Takemura, Yoshitaka Shirasaki, Michihiro Takahama, Yoshihiko Noguchi, Kenta Ikoma, Yixi Pan, Shuhei Nishida, Manabu Taura, Akiyoshi Nakayama, Takashi Funatsu, Takuma Misawa, Yoshie Harada, Toshiaki Sunazuka, Tatsuya Saitoh

International Immunology, dxac028 

■Cell-autonomous control of intracellular temperature by unsaturation of phospholipid acy1 chains

Akira Murakami,Kohjiro Nagao,Reiko Sakaguchi,Keisuke Kida,Yuji Hara,Yasuo Mori,Kohki Okabe,Yoshie Harada,Masato Umeda

Cell Report (March 15, 2022) 

2021

■Microscopic temperature control reveals cooperative regulation of actin–myosin interaction by drebrin E

Hiroaki Kubota†, Hiroyuki Ogawa†, Makito Miyazaki†, Shuya Ishii, Kotaro Oyama, Yuki Kawamura, Shin’ichi Ishiwata*, and Madoka Suzuki*

Nano Letters, 21, 9526-9533 (2021). 

■Opportunities for hybrid diamond nanosensors targeting photothermal applications in biological systems

Madoka Suzuki*, and Taras Plakhotnik*

Applied Physics Letters, 119, 190502 (2021). 

■Pressure-induced changes on the morphology and gene expression in mammalian cells.

Kazuko Okamoto , Tomonobu M. Watanabe, Masanobu Horie, Masayoshi Nishiyama, Yoshie Harada, and Hideaki Fujita

Biology Open, 10, bio058544 (2021). 

■In situ measurement of intracellular thermal conductivity using heater-thermometer hybrid diamond nanosensors.

Shingo Sotoma, Chongxia Zhong (co-first), James Chen Yong Kah, Hayato Yamashita, Taras Plakhotnik*, Yoshie Harada* and Madoka Suzuki*

Science Advances, 7, eabd7888 (2021). 

2020

■Tracking the 3D Rotational Dynamics in Nanoscopic Biological Systems.

Ryuji Igarashi*, Takuma Sugi, Shingo Sotoma, Takuya Genjo, Yuta Kumiya, Erik Walinda, Hiroshi Ueno, Kazuhiro Ikeda, Hitoshi Sumiya, Hidehito Tochio, Yohsuke Yoshinari, Yoshie Harada* and Masahiro Shirakawa*

Journal of the American Chemical Society, 142, 7542-7554 (2020). 

■In situ measurement of intracellular thermal conductivity using heater-thermometer hybrid diamond nanosensor.

Shingo Sotoma, Chongxia Zhong, James Chen Yong Kah, Hayato Yamashita, Taras Plakhotnik*, Yoshie Harada* and Madoka Suzuki*

bioRxiv, https://doi.org/10.1101/2020.06.03.126789. 

■The challenge of intracellular temperature.

Madoka Suzuki* and Taras Plakhotnik

Biophysical Reviews, 142, 593-600 (2020). 

2019

■Multifunctional temozolomide-loaded lipid superparamagnetic nanovectors: Dual targeting and disintegration of glioblastoma spheroids by synergic chemotherapy and hyperthermia treatment.

Attilio Marino, Alice Camponovo, Andrea degl’Innocenti, Martina Bartolucci, Christos Tapeinos, Chiara Martinelli, Daniele de Pasquale, Francesca Santoro, Valentina Mollo, Satoshi Arai, Madoka Suzuki, Yoshie Harada, Andrea Petretto and Gianni Ciofani

Nanoscale, 11, 21227-21248 (2019)

doi: 10.1039/C9NR07976A.

■Polydopamine Coating as a Scaffold for Ring-Opening Chemistry To Functionalize Gold Nanoparticles.

Shingo Sotoma* and Yoshie Harada

Langmuir. 35, 8357-8362 (2019).

doi: 10.1021/acs.langmuir.9b00762. (link) 

■HCV IRES captures an actively translating 80S ribosome.

Takeshi Yokoyama, Kodai Machida†, Wakana Iwasaki†, Tomoaki Shigeta, Madoka Nishimoto, Mari Takahashi, Ayako Sakamoto, Mayumi Yonemochi, Yoshie Harada, Hideki Shigematsu, Mikako Shirouzu, Hisashi Tadakuma*, Hiroaki Imataka* and Takuhiro Ito*

Molecular Cell. 74(6), 1205-1214 (2019).

doi: 10.1016/j.molcel.2019.04.022. 

2018

■Magnetic Field Imaging of Superparamagnetic Particles Using High-Density, Perfectly Oriented NV Centers in Diamond CVD Film.

Yuji Hatano*, Takeharu Sekiguchi, Takayuki Iwasaki, Mutsuko Hatano and Yoshie Harada

Physica Status Solidi (a), 215(22), pssa.201800254 (2018).

doi: 10.1002/pssa.201800254  

■Fluorescent nanodiamonds as a robust temperature sensor inside a single cell.

Takeharu Sekiguchi, Shingo Sotoma† and Yoshie Harada*

Biophysics and Physicobiology, 15, 229-234 (2018),

doi: 10.2142/biophysico.15.0_229 

■Construction of integrated gene logic-chip.

Takeya Masubuchi, Masayuki Endo*, Ryo Iizuka, Ayaka Iguchi, Dong Hyun Yoon, Tetsushi Sekiguchi, Hao Qi, Ryosuke Iinuma, Yuya Miyazono, Shuichi Shoji, Takashi Funatsu, Hiroshi Sugiyama*, Yoshie Harada, Takuya Ueda* and Hisashi Tadakuma*

Nature Nanotechnology. 13(10), 933-940 (2018).

doi: 10.1038/s41565-018-0202-3. 

■Enrichment of ODMR-active nitrogen-vacancy centres in five-nanometre-sized detonation-synthesized nanodiamonds: Nanoprobes for temperature, angle and position.

Shingo Sotoma, Daiki Terada, Takuya F. Segawa, Ryuji Igarashi*, Yoshie Harada* and Masahiro Shirakawa*

Scientific Reports, 8(1), pp 5463 (2018),

doi: 10.1038/s41598-018-23635-5 

■Commonly stabilized cytochromes c from deep-sea Shewanella and Pseudomonas.

Sotaro Fujii, Misa Masanari-Fujii, Shinya Kobayashi, Chiaki Kato, Masayoshi Nishiyama, Yoshie Harada, Satoshi Wakai and Yoshihiro Sambongi*

Biosci Biotechnol Biochem., 82(5), pp 792-799 (2018),

doi: 10.1080/09168451.2018.1448255 

■Morphological Control of Microtubule-Encapsulating Giant Vesicles by Changing Hydrostatic Pressure.

Kingo Takiguchi*, Masahito Hayashi, Yuki Kazayama, Taro Toyota, Yoshie Harada and Masayoshi Nishiyama

Biol Pharm Bull., 41(3), pp 288-293 (2018),

doi: 10.1248/bpb.b17-00366  

2017

■Live Cell Imaging Using Photoswitchable Diarylethene-Doped Fluorescent Polymer Dots.

Yasuko Osakada*, Tuyoshi Fukaminato*, Yuma Ichinose, Mamoru Fujitsuka*, Yoshie Harada and Tetsuro Majima*

Chem Asian J., 12(20), pp 2660-2665 (2017),

doi: 10.1002/asia.201701038 

2016

■Preferential 5-Methylcytosine Oxidation in the Linker Region of Reconstituted Positioned Nucleosomes by Tet1 Protein.

Seiichiro Kizaki, Tingting Zou, Yue Li, Yong‐Woon Han, Yuki Suzuki, Yoshie Harada and Hiroshi Sugiyama*

Chemistry, 22(46), pp 16598-16601 (2016),

doi: 10.1002/chem.201602435 

■Reversible Morphological Control of Tubulin-Encapsulating Giant Liposomes by Hydrostatic Pressure

Masahito Hayashi, Masayoshi Nishiyama, Yuki Kazayama, Taro Toyota, Yoshie Harada and Kingo Takiguchi*

Langmuir , 32 (15), pp 3794–3802 (2016), doi:10.1021/acs.langmuir.6b00799 

■The application of fluorescence-conjugated pyrrole/imidazole polyamides in the characterization of protein–DNA complex formation

Yong-Woon Han*, Hiroshi Sugiyama* and Yoshie Harada*

Biomater. Sci , Advance Article (2016), doi:10.1039/C5BM00214A 

2015

■Synergistic effect of ATP for RuvA-RuvB-Holiday junction DNA complex formation.

Takuma Iwasa†, Yong-Woon Han†, Ryo Hiramatsu, Hiroaki Yokota, Kimiko Nakao, Ryuji Yokokawa, Teruo Ono and Yoshie Harada*

Scientific Reports , 5, 18177 (2015), doi:10.1038/srep18177 

■Suppression of Nonspecific Protein–Nanodiamond Adsorption Enabling Specific Targeting of Nanodiamonds to Biomolecules of Interest

Shingo Sotoma, Ryuji Igarashi*, Jun Iimura, Yuta Kumiya, Hidehito Tochio, Yoshie Harada and Masahiro Shirakawa*

Chemistry Letters , 44(3), 354-356 (2015), doi:org/10.1246/cl.141036  

■ECHO-liveFISH: in vivo RNA labeling reveals dynamic regulation of nuclear

RNA foci in living tissues Nucl.

Ikumi Oomoto, Asuka Suzuki-Hirano, Hiroki Umeshima, Yong-Woon Han, Hiroyuki Yanagisawa, Peter Carlton, Yoshie Harada, Mineko Kengaku, Akimitsu Okamoto, Tomomi Shimogori and Dan Ohtan Wang*

Nucleic Acids Res., 43(19), e126 (2015), doi:10.1093/nar/gkv614  

■Comprehensive and quantitative analysis for controlling the physical/chemical states and particle properties of nanodiamonds for biological applications.

Shingo Sotoma, Kenichi Akagi, Saburo Hosokawa, Ryuji Igarashi, Hideto Tochio, Yoshie Harada and Masahiro Shirakawa*

RSC Adv., 5, 13818-13827 (2015), doi:10.1039/C4RA16482B 

■Optically Detected Magnetic Resonance of Nanodiamonds In Vivo; Implementation of Selective Imaging and Fast Sampling.

Yosuke Yoshinari*, Shigeyuki Mori, Ryuji Igarashi, Takuma Sugi, Hiroaki Yokota, Kazuhiro Ikeda, Hitoshi Sumiya, Ikue Mori, Hidehito Tochio, Yoshie Harada* and Masahiro Shirakawa

Journal of Nanoscience and Nanotechnology , 15,1014-1021(2015), doi:10.1166/jnn.2015.9739 

2014

■Quantitative In Vivo Fluorescence Cross-Correlation Analyses Highlight the Importance of Competitive Effects in the Regulation of Protein-Protein Interactions.

Wakako Sadaie, Yoshie Harada, Michiyuki Matsuda and Kazuhiro Aoki*

Molecular and Cellular Biology , 34(17), 3272-3290 (2014), doi:10.1128/MCB.00087-14 

■Effective Production of Fluorescent Nanodiamond Containing Negatively-Charged Nitro-gen-Vacancy Center by Ion Irradiation.

Shingo Sotoma, Yosuke Yoshinari, Ryuji Igarashi, Akiyoshi Yamazaki, Shige H. Yoshimura, Hidesato Tochio, Masahiro Shirakawa and Yoshie Harada*  

■Real-time single-cell imaging of protein secretion.

Yoshitaka Shirasaki, Mai Yamagishi, Nobutake Suzuki, Kazushi Izawa, Asahi Nakahara, Jun Mizuno, Shuichi Shoji, Toshio Heike, Yoshie Harada, Ryuta Nishikomori and Osamu Ohara*

Scientific Reports, 4, 4736 (2014), doi:10.1038/srep04736 

■Hard X-ray-induced optical luminescence via　biomolecule-directed metal clusters.

Yasuko Osakada*, Guillem Pratx, Conroy Sun, Masanori Sakamoto, Moiz Ahmad, Olga Volotskova, Qunxiang Ong, Toshiharu Teranishi, Yoshie Harada and Lei Xing* and Bianxiao Cui*

Chemical Communications(Camb), 50, 3549-3551 (2014), doi:10.1039/c3cc48661c 

■Construction and Characterization of Cy3- or Cy5-Conjugated Hairpin Pyrrole/Imidazole Polyamides binding to DNA in the Nucleosome.

Yong-Woon Han*, Yasuo Tsunaka, Hiroaki Yokota, Tomoko Matsumoto, Gengo

Kashiwazaki, Hironobu Morinaga, Kaori Hashiya, Toshikazu Bando, Hiroshi Sugiyama* and Yoshie Harada*

Biomaterials Science , 2 ,297-307 (2014), doi:10.1039/C3BM60202H