Results

The receptors aligned using MUSCLE are listed below, with numbers in the table corresponding to numbers in the matrix. Following is the percent identity matrix generated from the alignment. Matching pairs refer to whether pairs are in the same of the three groups: human receptors that function in yeast, human receptors that don't function in yeast, and yeast receptors.

EV mutation analysis was used to rank the five most deleterious mutations at each GPCR with the rationale that location of disadvantageous mutation could provide insight into where receptors are most vulnerable to change in function. For example, if sterol composition was the determining factor for heterologous GPCR expression, amino acids of transmembrane domains might be less mutable than soluble sequences. Scores at each location for each receptor were summed, and the five most negative scores for each receptor are shown below. Due to constrains in the EV Couplings server, only the GPCRs listed below were able to be analyzed using the EV strategies. There was no consensus location as to most or least mutable.

Predicted structures of the GPCRs listed below were analyzed in Chimera. The Match Align tool was used to map distances between residues of the superimposed structures. The alignment is shown below. There was no column with alignments from all of the superimposed GPCRs, and no enrichment of aligned residues from GPCRs that function in yeast.