None of the analyzes proved to be predictive or even descriptive of the ability to functionally express human GPCRs in yeast. These strategies consisted of analysis of primary protein structure, analysis of alignment of tertiary structure, and location of predicted deleterious mutations. A large caveat of this work is the small sample size. The data set was limited for a number of reasons. First, there is not a comprehensive list of human GPCRs that have experimentally been shown to function in yeast. I compiled a list myself from a literature search, with preference for receptors without a solved structure. Second, as most groups do not publish negative results, I was limited to the receptors shown to not function in yeast from my lab. This limited me to two receptors for comparison to the functional receptors. Third, the EV Couplings server crashed for some of the proteins, most likely indicating an issue with either the alignment or the complex pipelines. These pipelines can be manually tweaked, but this is not something I did for the project, and my data set was therefore limited further for the tertiary structure analysis. In addition, I got error messages for proteins that were too large, again limiting my data set for this analysis. For any sound conclusions to be made about the effect of these analyzes on their ability to predict expression of human GPCRs in yeast, a much larger and more comprehensive data set would need to be used.