Toopy is a funny, friendly, optimistic, impulsive mouse whose insatiable zest for life is matched only by his love for his best friend, Binoo, a stuffed cat who is logical, sensible, and thinks before he acts. Binoo is devoted to his best friend Toopy. The characters are charming and endearing. The kindness, respect, and gentle aspects of childhood friendship are emphasized as the friends explore and discover the world around them with their colorful adventures. Toopy and Binoo allows for learning in a non-didactic manner. Individual segments are approximately five minutes in length, but five of them are frequently paired together into one 24-minute episode, both on television and DVD releases.

There are also short 2-minute episodes that are seen on the website in the second season, where, there are either "Magic You", "Captain You", or "Fabulous You" segments, featuring Toopy and Binoo as space captains, fairies, or, as superheroes who make things right by using magic (Toopy sometimes ends it by doing the same thing that's wrong on himself which he doesn't even notice), and they use the magic wand from "Godmother Toopy", explore things in their house and pretend to find out what they are, and, Binoo sometimes reveals what they are, such as a pillow, where, they use the same uniforms they had in "Strange New World", but, instead, the object they find is the entire form of the planet, and, help their friends when they have nothing to play with by playing games they can use with themselves. They use the same uniforms they had in "Super Toopy", however, Binoo does strikingly resemble Super Toopy. The segments encourage viewers (referred to in the show as "Magic You", "Captain You", or, "Fabulous You") to participate in the adventures and use their imaginations. Each DVD that has two half-hour specials has two of each of these three short mini-episode adventures, starting in this order: "Magic You", "Captain You", and then, "Fabulous You".