Research articles of the SEPTIMORF consortium are listed below.
Human septins organize as octamer-based filaments and mediate actin-membrane anchoring in cells
Carla Silva Martins, Cyntia Taveneau, Gerard Castro-Linares, Mikhail Baibakov, Nicolas Buzhinsky, Mar Eroles, Violeta Milanović, Shizue Omi, Jean-Denis Pedelacq, Francois Iv, Léa Bouillard, Alex Llewellyn, Maxime Gomes, Mayssa Belhabib, Mira Kuzmić, Pascal Verdier-Pinard, Stacey Lee, Ali Badache, Sanjay Kumar, Cristel Chandre, Sophie Brasselet, Felix Rico, Olivier Rossier, Gijsje H. Koenderink, Jerome Wenger, Stéphanie Cabantous, Manos Mavrakis
Journal of Cell Biology 222 (3), e202203016 (2023)
Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins
Brieuc Chauvin, Koyomi Nakazawa, Alexandre Beber, Aurélie Di Cicco, Bassam Hajj, François Iv, Manos Mavrakis, Gijsje H. Koenderink, João T. Cabral, Michaël Trichet, Stéphanie Mangenot, Aurélie Bertin
Journal of Visualized Experiments 186, e63889 (2022)
Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution
Gerard Castro-Linares, Jeffrey den Haan, Francois Iv, Carla Silva Martins, Aurélie Bertin, Manos Mavrakis, Gijsje H. Koenderink
Journal of Visualized Experiments 184, e63871 (2022)
4polar-STORM polarized super-resolution imaging of actin filament organization in cells
Caio Vaz Rimoli, Cesar Augusto Valades Cruz, Valentina Curcio, Manos Mavrakis and Sophie Brasselet
Nature Communications 13, 301 (2022)
Septin-microtubule association requires a MAP-like motif unique to Sept9 isoform 1 embedded into septin octamers
Mira Kuzmić, Gerard Castro Linares, Jindřiška Leischner Fialová, François Iv, Danièle Salaün, Alex Llewellyn, Maxime Gomes, Mayssa Belhabib, Yuxiang Liu, Keisuke Asano, Magda Rodrigues, Daniel Isnardon, Taro Tachibana, Gijsje Koenderink, Ali Badache, Manos Mavrakis, Pascal Verdier-Pinard
Jounal of Cell Science 135(1): jcs.258850 (2022)
Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms
Francois Iv, Carla Silva Martins, Gerard Castro-Linares, Cyntia Taveneau, Pascale Barbier, Pascal Verdier-Pinard, Luc Camoin, Stéphane Audebert, Feng-Ching Tsai, Laurie Ramond, Alex Llewellyn, Mayssa Belhabib, Koyomi Nakazawa, Aurélie Di Cicco, Renaud Vincentelli, Jerome Wenger, Stéphanie Cabantous, Gijsje H. Koenderink, Aurélie Bertin, Manos Mavrakis
Jounal of Cell Science 134 (15): jcs258484 (2021)
Membrane binding controls ordered self-assembly of animal septins
Szuba, A., Bano, F., Iv, F., Mavrakis, M., Richter, R.P., Bertin, A. and Gijsje H. Koenderink
eLife 10:e63349 (2021)
Septin-related research articles from the partners of the consortium prior to the ANR Septimorf are listed below.
High-content tripartite split-GFP cell-based assays to screen for modulators of small GTPase activation.
Koraïchi F, Gence R, Bouchenot C, Grosjean S, Lajoie-Mazenc I, Favre G, Cabantous S.
J Cell Sci. 8;131(1) (2018)
Septin 9_i2 is downregulated in tumors, impairs cancer cell migration and alters subnuclear actin filaments.
Verdier-Pinard, P., Salaun, D., Bouguenina, H., Shimada, S., Pophillat, M., Audebert, S., Agavnian, E., Coslet, S., Charafe-Jauffret, E., Tachibana, T. and A. Badache
Scientific Reports 7, 44976 (2017)
Preparing recombinant yeast septins and their analysis by electron microscopy.
Bertin A, Nogales E.
Methods in Cell Biology, Volume 136, 21-34 (2016)
Visualizing septins in early Drosophila embryos.
Mavrakis, M.
Methods in Cell Biology, Volume 136, 199-220 (2016)
Purification of recombinant human and Drosophila septin hexamers for TIRF assays of actin-septin filament assembly.
Mavrakis, M., Tsai, F.-C., and Koenderink, G.H.
Methods in Cell Biology, Volume 136, 183-198 (2016)
Cytoskeletal crosstalk: when three different personalities team up.
F. Huber, A. Boire, M.P. Lopez, G.H. Koenderink
Curr. Opin. Cell Biol. 32: 39-47 (2015)
In vitro reconstitution of dynamic microtubules interacting with actin filament networks.
Preciado López M, Huber F, Grigoriev I, Steinmetz MO, Akhmanova A, Dogterom M, Koenderink GH.
Methods Enzymol. 540:301-20 (2014)
Septins promote F-actin ring formation by cross-linking actin filaments into curved bundles.
Mavrakis, M., Azou-Gros, Y., Tsai, F.-C., Alvarado, J., Bertin, A., Iv, F., Kress, A., Brasselet, S., Koenderink, G.H. and T. Lecuit
Nature Cell Biology 16 (4), 322-334 (2014)
(rated as "exceptional" by F1000Prime; featured in Curr. Biol. (2014) 24(11), R526)
Actin-microtubule coordination at growing microtubule ends.
M. Preciado Lopez, F. Huber, I. Grigoriev, M.O. Steinmetz, A. Akhmanova, G.H. Koenderink, M. Dogterom
Nature Communications 5:4778 (2014)
A new protein-protein interaction sensor based on tripartite split-GFP association.
Cabantous S, Nguyen HB, Pedelacq JD, Koraïchi F, Chaudhary A, Ganguly K, Lockard MA, Favre G, Terwilliger TC, Waldo GS.
Sci Rep. 4;3:2854. (2013)
Three-dimensional ultrastructure of the septin filament network in Saccharomyces cerevisiae.
Bertin, A., M. McMurray, J. Pierson, L. Thai, E. Zehr, K. McDonald, J. Thorner, E. Nogales
Molecular Biology of the Cell, 23(3), 423-432 (2012)
Septin roles in tumorigenesis.
Connolly, D., Abdesselam, I., Verdier-Pinard, P. and C Montagna
Biol Chem,. 392:725-38 (2011)
Septin 9 isoform expression, localization and epigenetic changes during human and mouse breast cancer progression.
Connolly, D., Yang, Z., Castaldi, M., Simmons, N., Oktay, M.H., Coniglio, S., Fazzari, M.J., Verdier-Pinard, P. and C. Montagna
Breast Cancer Res. 13:R76. (2011)
Phosphatydinositol 4,5 biphosphate promotes budding yeast septin filament assembly and organization.
Bertin, , M. McMurray, L. Thai , G. Garcia, V. Votin, P. Grob, T. Allyn, J. Thorner, E. Nogales
J. Mol. Biol., 404(4), 711-31 (2010)
Saccharomyces Cerevisiae septins: supramolecular organization of heterooligomers and the mechanism of filament assembly.
A.Bertin, P. Grob, M. McMurray, G.Garcia, S. Park, H. Ng, T. Alber, J. Thorner, E. Nogales
Proc.Natl. Acac. Sci USA, 105, 8274-8279 (2008)
Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein.
Cabantous, S., Terwilliger, T.C. & Waldo, G.S.
Nature Biotechnology, 23 (1):102-7 (2005)