海洋直刺 Mar Plant

Marine Plant Lab
Department of Marine Biotechnology and ResourcesNational Sun Yat-sen University Kaohsiung 80424, Taiwan
886-7-5252000 ext. 5110; 886-7-5251509
Email: tmlee@mail.nsysu.edu.tw
Chinese website: https://sites.google.com/view/leealgae51/Home

Marine Plant Stress Molecular Physiology and Ecology: 😍A Gateway to Climate Change Resilience

Our laboratory explores how microalgae and macroalgae enhance stress resilience through metabolic regulation, enabling adaptation to climate-induced challenges. We focus on their dual role in CO₂ capture and sustainable waste treatment, transforming nutrient-rich effluents into valuable bioproducts. This research supports algae-based solutions for carbon sequestration, resource recycling, and circular bioeconomy development.

Our lab also explores how coastal ecosystems contribute to long-term carbon storage through what are known as “blue carbon” systems, including mangroves, salt marshes, and seagrasses. While these habitats are well-known for their ability to sequester organic carbon, much of that carbon may come from outside sources. One key, yet often overlooked, contributor is the blooming of macroalgae due to anthropogenic activities and runoff from terrestrial plant detritus caused by heavy precipitation resulting from climate change. With their high growth rates, tendency to fragment, and capacity for transport across ecosystems, macroalgae can play a significant role in supplying carbon to these blue carbon sinks. To better understand how stable macroalgal tissues are—and how likely they are to contribute to long-term carbon storage—we use eDNA, isotope C and N, and thermogravimetric analysis (TGA) to assess carbon stability across a range of marine macroalgae and coastal plant species.

Research Areas
- Algal stress physiology and carbon metabolism
- Cryptic Carbon: Utilizing environmental DNA (eDNA) alongside lignin and lipid biomarker analyses to monitor and assess hidden carbon sinks in both coastal and open-ocean systems (Macroalgae (Seaweeds) and Terrestrial Plants)
- Net-zero carbon emission strategies: Develop nature-based and human-assisted solutions
Ongoing Projects
- TRANSCRIPTION FACTORS in algal starch metabolism and ascorbate-glutathione cycle
- eDNA TRACKING, LIGIN AND HUMIC ACID QUANTATIVE DETECTION, and EMPIRICAL MODELLING of macrophyte carbon in the South China Sea and low-latitude Northwestern Pacific
- Algae-, fish-, and microbe-based zero-waste carbon reduction systems in AQUACULTURE

https://doi.org/10.1016/j.biortech.2024.131929AbstractAquaculture waste and carbon dioxide (CO2) are a global concern. Sarcodia suae cultivated in outdoor photobioreactors (PBRs) using shrimp aquaculture wastewater and CO2 from biogas demonstrates significant benefits. It boosted biomass by 18.72% with nearly 100% removal of COD, inorganic nitrogen (N), phosphorus (P), and 41-89% organic N and P removal from AWW. Sarcodia aqueous extracts were biostimulants for promoting mung bean and rice seedling growth, and Sarcodia remnants/shrimp pond bottom sludge generated a biogas yield of 457.07 L/kg-VS methane (CH4)/day. Biogas provides renewable energy for the system's needs. The solid residues after biogas fermentation serve as biofertilizers for enhancing rice yield by a 15% increase up to 5.53 tons/ha/year. A Zero-Waste approach is successfully established using S. suiae as a model for recirculating aquaculture wastes to sequester 239.04 tons CO2/ha/year, capture N and sulfur (S), and produce renewable energy. and create biostimulants and biofertilizers.

Hung, C. C., Chang, J. S., Liao, C. H., & Lee, T. M. (2024). Exploring the impact of ocean warming and nutrient overload on macroalgal blooms and carbon sequestration in deep-sea sediments of the subtropical western North Pacific. Marine pollution bulletin, 208, 116918. https://doi.org/10.1016/j.marpolbul.2024.116918

Chang, Y. E., Liao, C. H., Hsieh, H. H., Gradngay Abedneko, V., Hung, C. C., & Lee, T. M. (2024). Lateral carbon export of macroalgae and seagrass from diverse habitats contributes particulate organic carbon in the deep sea of the Northern South China Sea. Marine pollution bulletin, 206, 116672. https://doi.org/10.1016/j.marpolbul.2024.116672
Later transport of land plants, including coastal species, like seagrass and mangrove, and seaweed, to several hundred km off Dongsha island and southeastern Taiwan in the SCS due to the current jet and sinking

Environmental DNA (eDNA) 18S V9 rDNA metabarcoding of marine macrophytes (SEAWEED, SEAGRASS, MANGROVE) in deep-sea shelf, slope, canyon, and seafloor in Palau: Seaweed Carbon Sequestration in the Western North Pacific

https://maps.coastalresilience.org/palau/#/

Environmental DNA (eDNA) 18S V9 rDNA metabarcoding of marine macrophytes (SEAWEED, SEAGRASS, MANGROVE) in deep-sea shelf, slope, canyon, and seafloor in South China Sea: Sseaweed and Seagrass Carbon Sequestration in the Western North Pacific marginal sea

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Tse-Min Lee, Jia-Yi Lin, Tsung-Han Tsai, Ru-Yin Yang, I-Son Ng, 2023. Clustered regularly interspaced short palindromic repeats (CRISPR) technology and genetic engineering strategies for microalgae towards carbon neutrality: A critical review, Bioresource Technology, 368, 128350, ISSN 0960-8524, https://doi.org/10.1016/j.biortech.2022.128350. (https://www.sciencedirect.com/science/article/pii/S0960852422016832)Abstract: Carbon dioxide is the major greenhouse gas and regards as the critical issue of global warming and climate changes. The inconspicuous microalgae are responsible for 40% of carbon fixation among all photosynthetic plants along with a higher photosynthetic efficiency and convert the carbon into lipids, protein, pigments, and bioactive compounds. Genetic approach and metabolic engineering are applied to accelerate the growth rate and biomass of microalgae, hence achieve the mission of carbon neutrality. Meanwhile, CRISPR/Cas9 is efficiently to enhance the productivity of high-value compounds in microalgae for it is easier operation, more affordable and is able to regulate multiple genes simultaneously. The genetic engineering strategies provide the multidisciplinary concept to evolute and increase the CO2 fixation rate through Calvin–Benson–Bassham cycle. Therefore, the technologies, bioinformatics tools, systematic engineering approaches for carbon neutrality and circular economy are summarized and leading one step closer to the decarbonization society in this review.