Polyelectrolyte Complexation

We are building a comprehensive understanding of the molecular factors that govern polyelectrolyte complexation with nucleic acids, with the primary goal of using polyelectrolyte complex micelles (PCMs) for therapuetics. These nanoparticles are formed by the self-assembly of oppositely charged polymers and cargo molecules, which can be various biomolecules (i.e. therapeutic nucleic acids). Their highly tunable properties enable controlled encapsulation, protection, and intracellular delivery of nucleic acids, making them attractive candidates for next-generation therapeutic applications. 

Lipid Nanoparticles (LNPs)

We are developing structure–property-function relationships to enable the rational design of lipid nanoparticles (LNPs). LNPs are nanoscale delivery vehicles composed of ionizable lipids, helper phospholipids, cholesterol, and PEG-lipids that self-assemble with therapeutic cargo. LNPs have emerged as a clinically validated platform and have been widely utilized in mRNA vaccines and gene therapies. Their modular composition and highly tunable physicochemical properties enable efficient encapsulation, protection from degradation, and targeted intracellular delivery of nucleic acids.

DNA Origami Mechanisms

Using DNA as a construction material, we design dynamic and controllable DNA-based nanomechanisms by applying engineering approaches used to make macroscopic machines to the nanoscale. We program our dynamic DNA devices to react to molecular signals, triggering an optical readout or material property change in realtime.