Storage Buffer Composition Impacts Internal Structure, Freeze-Thaw Stability, and Transfection Efficiency of mRNA-Lipid Nanoparticles

Mohammadi-Zerankeshi, M, Charland, D., Donnelly, K., Nash, G., Shi, J., Patil, D., Ennis, J., Rodriguez, K.G., Corba, S., Wambolt, N., AboulFotouh, K., Kawelah, M.R., Oh, Y., Yang, D., Qian, K., Cui, Q., Johnston, K.P., Estabrook, D., Marras, A.E.

ACS Nano

Impact of DNA Sequence and Structure on Polyelectrolyte Complex Micelle Morphology

Hussain, A.F., Keys, C., Houston, J., Marras, A.E.

Chemrxiv

Structural heterogeneity in mRNA‑LNP subpopulations revealed by AF4‑SAXS: implications for cargo loading and cell transfection

Sanchez-Fernandez, A*, Donnelly, K.A. *, Bolinsson, H., Börjesdotter, A.M., Bobak, T.R., Erlendsson, S., Mohammadi‐Zerankeshi, M., AboulFotouh, K., Kawelah, M.R., Herranz-Trillo, F., Schagerlöf, H., Palmiero, U.C., Huus, K., Johnston, K.P., Marras, A.E., Nilsson, L.

Biorxiv

Unraveling the folding dynamics of DNA origami structures

Mohammadi Zerankeshi, M., Houston, J., Elisha-Wigwe, O. K.U., Sachi, A., Marras, A.E. 

Small

Systematic screening of excipients to stabilize aerosolized lipid nanoparticles for enhances mRNA delivery

Heiser, B.J., Lewis, M.M., Mohammadi Zerankeshi, M., Netemeyer, E., Hernandez, A., Marras, A.E., Ghosh, D.  

Royal Society of Chemistry Pharmaceutics

Growth of clusters towards liquid-liquid phase separation of monoclonal antibodies as characterized by small angle X-ray scattering and molecular dynamics simulation

Kimball, W.D., Lanzaro, A., Hurd, C., Jhaveri, N., Huang, J., Lewandowski, J., Qian, K.K., Woldeyes, M.A., Majumdar, R., Witek, M.A., Feng, J., Gillilan, R.E., Huang, Q., Marras, A.E., Truskett, T.M., Johnston, K. P. 

Journal of Physical Chemistry B

Characterization of mRNA lipid nanoparticles by electron density mapping reconstruction: X-ray scattering with density from solution scattering (DENSS) algorithm

Dao, H.M., AboulFotouh, K., Hussain, A.F., Marras, A.E., Johnston, K.P., Cui, Z., Williams, R.O. 

Pharmaceutical Research

Antibody conjugated polymersomes with encapsulated indocyanine green J-aggregates and high near-infra red (NIR) absorption for molecular photoacoustic cancer imaging

Kawelah, M.R., Han, S., Dincer, C.A., Jeon, J., Brisola, J., Hussain, A.F., Soundaram, A.J., Bouchard, R., Marras, A.E, Truskett, T.M., Sokolov, K.V., Johnston, K.P. 

ACS Applied Materials & Interfaces

Effect of charged block length mismatch on double diblock polyelectrolyte complex micelle cores

Stevens, K.C., Marras, A.E., Campagna, T.R., Ting, J.M., Tirrell, M.V. 

Macromolecules - pdf

Hierarchical assembly of DNA origami nanostructures

Marras, A.E.  

MRS Communications - pdf

Translocation behaviors of synthetic polyelectrolytes through alpha-Hemolysin (α-HL) and Mycobacterium smegmatis Porin A (MspA) nanopores

Wang, X., Stevens, K.C., Ting, J.M., Marras, A.E., Rezvan, G., Wei, X., Taheri-Qazvini, N., Tirrell, M.V., Liu, C. 

Journal of the Electrochemical Society

Advances in the structural design of polyelectrolyte complex micelles

Marras, A.E., Ting, J.M., Stevens, K.C., Tirrell, M.V. 

Journal of Physical Chemistry B - pdf

Physical property scaling relationships for polyelectrolyte complex micelles

Marras, A.E., Campagna, T.R., Vieregg, J.R., Tirrell, M.V. 

Macromolecules

Impact of wet-dry cycling on the phase behavior and compartmentalization properties of complex coacervates

Fares, H.M., Marras, A.E., Ting, J.M., Tirrell, M.V., Keating, C.D. 

Nature Communications - pdf

Comparing zwitterionic and PEG exteriors of polyelectrolyte complex micelles

Ting, J. M.*, Marras, A. E.*, Mitchell, J. D., Campagna, T., Tirrell, M.V. 

Molecules - pdf

Assembly and characterization of polyelectrolyte complex micelles

Marras, A.E., Vieregg, J.R., Tirrell, M.V.  

Journal of Visualized Experiments

Polyelectrolyte complexation of oligonucleotides by charged hydrophobic – neutral hydrophilic block copolymers

Marras, A.E., Vieregg, J.R., Ting, J.M., Rubien, J.D., Tirrell, M.V.  

Polymers - pdf

Paper origami-inspired design and actuation of DNA nanomachines with complex motions

Zhou, L., Marras, A.E., Huang, C.M., Castro, C.E., Su, H-J. 

Small - pdf

Cation-activated avidity for rapid reconfiguration of DNA nanodevices

Marras, A.E., Shi, Z., Lindell, M., Patton, R.A., Huang, C.M., Zhou, L., Su, H-J., Arya, G., Castro, C.E. 

ACS Nano

Real-time magnetic actuation of DNA nanodevices via modular integration with stiff micro-levers

Lauback, S., Mattioli, K., Marras, A.E., Armstrong, M., Rudibaugh, T., Sooryakumar, R., Castro, C.E. 

Nature Communications

Three-dimensional structural dynamics of DNA origami Bennett linkages using individual-particle electron tomography

Lei, D., Marras, A.E., Liu, J., Huang, C.M., Zhou, L., Castro, C.E., Su, H.J., Ren, G. 

Nature Communications

The kinematic principle for designing deoxyribose nucleic acid origami mechanisms: challenges and opportunities

Su H-J, Castro C.E., Marras A.E., Zhou L. 

Journal of Mechanical Design

Projection kinematic analysis of DNA origami mechanisms based on a two-dimensional TEM image

Zhou, L., Su, H.J., Marras, A.E., Huang, C.M., Castro, C.E. 

Mechanism and Machine Theory

Directing folding pathways for multi-component DNA origami nanostructures with complex topology

Marras, A.E., Zhou, L., Kolliopoulos, V., Su, H.J., Castro, C.E. 

New Journal of Physics

Pseudorigid-Body Models of Compliant DNA Origami Mechanisms

Zhou, L., Marras, A.E., Castro, C.E., Su, H-J. 

Journal of Mechanisms and Robotics

Direct design of an energy landscape with bistable DNA origami mechanisms

Zhou, L., Marras, A.E., Su, H.J., Castro, C.E. 

Nano Letters

Mechanical Design of DNA nanostructures

Castro, C.E., Su, H.J., Marras, A.E., Zhou, L., Johnson, J.  

Nanoscale

Programmable motion of DNA origami mechanisms

Marras, A.E., Zhou, L., Su, H.J., Castro, C.E. 

Proceedings of the National Academy of Sciences

Research Highlights:

1. • • “Slider and Hinge Joints”, Nature Materials 14, 134 (2015) -- link

     • “On the Move”, Nature Nanotechnology 13 (2015) -- link

     • “DNA Origami Machine Elements with Complex Motion”, PNAS 112, 3 (2015) -- link

     • F1000 Prime Recommendation (2015) -- link

DNA origami compliant nanostructures with tunable mechanical properties

Zhou, L., Marras, A.E., Su, H.J., Castro, C.E.  

ACS Nano

Detection of Extracellular RNAs in Cancer and Viral Infection via Tethered Cationic Lipoplex Nanoparticles Containing Molecular Beacons

Wu, Y., Kwak, K.J., Agarwal, K., Marras, A., Wang, C., Mao, Y, Huang, X., Ma, J., Yu, B., Lee, R. Vachani, A., Marcucci, G., Byrd, J., Muthusamy, N., Otterson, G., Huang, K., Castro, C.E., Paulaitis, M., Nano-Sinkam, S.P., Lee, L.J. 

Analytical Chemistry