Publications from IIT Kanpur
Patil, D. N., S. Pantalone, Y. Cao, T. Laboute, S. J. Novick, S. Singh, S. Savino, S. Faravelli, F. Magnani, P. R. Griffin, A. K. Singh, F. Forneris, and K. A. Martemyanov. "Structure of the Photoreceptor Synaptic Assembly of the Extracellular Matrix Protein Pikachurin with the Orphan Receptor Gpr179." Sci Signal 16, no. 795 (Jul 25 2023): eadd9539. https://dx.doi.org/10.1126/scisignal.add9539.
Nageswar, Koomity V, Mansi Sharma, Dipak N Patil, Santoshi Nayak, Anwesha Roy, and Appu K Singh. "Current Trends in Membrane Protein Crystallography." In Advances in Protein Molecular and Structural Biology Methods: Academic Press, 2022.
Patil DN*, Singh S*, Laboute T, Strutzenberg TS, Qiu X, Wu D, Novick SJ, Robinson CV, Griffin PR, Hunt JF, Izard T, Singh, A.K#, Martemyanov KA#. Cryo-EM structure of human GPR158 receptor coupled to the RGS7-Gβ5 signaling complex. Science. (2021) Nov 18;:eabl4732. [Epub ahead of print] PubMed PMID: 34793198. PUBMED (#Co-Corresponding Author)
Nageswar, Koomity V, Santoshi Nayak, and Appu K Singh. "Trps and Piezos Receive the Spotlight for Winning the Nobel." Resonance 26 (2021): 1649-61.
Publications from postdoc and phd
2020
Bhardwaj R, Lindinger S, Neuberger A, Nadezhdin KD, Singh, A.K., Cunha MR, Derler I, Gyimesi G, Reymond JL, Hediger MA, Romanin C, Sobolevsky AI. Inactivation-mimicking block of the epithelial calcium channel TRPV6. Sci Adv. (2020) Nov;6(48). doi: 10.1126/sciadv.abe1508. PubMed PMID: 33246965; PubMed Central PMCID: PMC7695471. PUBMED
Yelshanskaya MV, Singh, A.K., Narangoda C, Williams RSB, Kurnikova MG, Sobolevsky AI. Structural basis of AMPA receptor inhibition by trans-4-butylcyclohexane carboxylic acid. Br J Pharmacol. (2020) Sep 22;. doi: 10.1111/bph.15254. [Epub ahead of print] PubMed PMID: 32959886. PUBMED
2019
ect
Singh AK, McGoldrick LL, Sobolevsky AI. Expression, Purification, and Crystallization of the Transient Receptor Potential Channel TRPV6. Methods Mol Biol. (2019) 1987:23-37. PubMed PMID: 31028671. PUBME
Singh, A.K., McGoldrick, L.L., Demirkhanyan, L., Leslie, M., Zakharian, E., and Sobolevsky, A.I. Structural basis of temperature sensation by the TRP channel TRPV3. Nature Structural & Molecular Biology (2019) (Featured as F1000 article). Pubmed
McGoldrick, L.L*., Singh, A.K*., Demirkhanyan, L., Lin, T.Y., Casner, R.G., Zakharian, E.,* and Sobolevsky, A.I. (2019). Structure of the thermo-sensitive TRP channel TRP1 from the alga Chlamydomonas reinhardtii. Nat Commun 10, 4180. Pubmed
2018
Singh, A. K*., McGoldrick, L. L*., Twomey, E. C., and Sobolevsky, A. I. (2018) Structure and gating mechanism of the transient receptor potential channel TRPV3." Nature Structural & Molecular Biology. (Featured as NSMB cover Page, and F1000 article). PUBMED
Singh, A. K*., McGoldrick, L. L*., and Sobolevsky, A. I. (2018) Mechanism of calmodulin inactivation of the calcium selective TRP channel TRPV6." Science Advances 4(8). PUBMED
Singh, A. K*., Saotome, K*., McGoldrick, L. L., and Sobolevsky, A. I. (2018) Structural bases of TRP channel TRPV6 allosteric modulation by 2-APB. Nat Commun 9, 2465. PUBMED
Singh, A. K., McGoldrick, L. L., Saotome, K., and Sobolevsky, A. I. (2018) X-ray crystallography of TRP channels. Channels (Austin) 12, 137-152. PUBMED
McGoldrick, L. L*., Singh, A. K*., Saotome, K., Yelshanskaya, M. V., Twomey, E. C., Grassucci, R. A., and Sobolevsky, A. I. (2018) Opening of the human epithelial calcium channel TRPV6. Nature 553, 233-237. PUBMED
2017
Singh, A. K*., Saotome, K*., and Sobolevsky, A. I. (2017) Swapping of transmembrane domains in the epithelial calcium channel TRPV6. Sci Rep 7, 10669. PUBMED
Singh, A. K*., Ekka, M. K*., Kaushik, A*., Pandya, V., Singh, R. P., Banerjee, S., Mittal, M., Singh, V., and Kumaran, S. (2017) Substrate-Induced Facilitated Dissociation of the Competitive Inhibitor from the Active Site of O-Acetyl Serine Sulfhydrylase Reveals a Competitive-Allostery Mechanism. Biochemistry 56, 5011-5025. PUBMED
Saotome, K., Singh, A. K., and Sobolevsky, A. I. (2017) Determining the Crystal Structure of TRPV6. in Calcium Entry Channels in Non-Excitable Cells, CRC Press. pp 275-292. PUBMED
Mittal, M., Singh, A. K., and Kumaran, S.(2017) Structural and biochemical characterization of ligand recognition by CysB, the master regulator of sulfate metabolism. Biochimie 142, 112-124. PUBMED
2016
Yelshanskaya, M. V., Singh, A. K., Sampson, J. M., Narangoda, C., Kurnikova, M., and Sobolevsky, A. I. (2016) Structural Bases of Noncompetitive Inhibition of AMPA-Subtype Ionotropic Glutamate Receptors by Antiepileptic Drugs. Neuron 91, 1305-1315. PUBMED
Yelshanskaya, M. V., Saotome, K., Singh, A. K., and Sobolevsky, A. I. (2016) Probing Intersubunit Interfaces in AMPA-subtype Ionotropic Glutamate Receptors. Sci Rep 6, 19082. PUBMED
Saotome, K*., Singh, A. K*., Yelshanskaya, M. V., and Sobolevsky, A. I. (2016) Crystal structure of the epithelial calcium channel TRPV6. Nature 534, 506-511. PUBMED
2011-2015
Singh, A. K*., Manjasetty, B*., Balasubramani, G. L., Koul, S., Kaushik, A., Ekka, M. K., Singh, V., and Kumaran, S. (2015) Crystal Structure of Fad35R from Mycobacterium tuberculosis H37Rv in the Apo-State. PLoS One 10, e0124333. PUBMED
Singh, A. K*., Singh, M*., Pandya, V. K., G, L. B., Singh, V., Ekka, M. K., Mittal, M., and Kumaran, S. (2014) Molecular basis of peptide recognition in metallopeptidase Dug1p from Saccharomyces cerevisiae. Biochemistry 53, 7870-7883. PUBMED
Biswas, D., Pandya, V., Singh, A. K., Mondal, A. K., and Kumaran, S. (2012) Co-factor binding confers substrate specificity to xylose reductase from Debaryomyces hansenii. PLoS One 7, e45525. PUBMED
Anand, S*., Singh, V*., Singh, A. K*., Mittal, M*., Datt, M., Subramani, B., and Kumaran, S. (2012) Equilibrium binding and kinetic characterization of putative tetracycline repressor family transcription regulator Fad35R from Mycobacterium tuberculosis. FEBS J 279, 3214-3228. PUBMED
Kaur, H., Datt, M., Ekka, M. K., Mittal, M., Singh, A. K., and Kumaran, S. (2011) Cys-Gly specific dipeptidase Dug1p from S. cerevisiae binds promiscuously to di-, tri-, and tetra-peptides: Peptide-protein interaction, homology modeling, and activity studies reveal a latent promiscuity in substrate recognition. Biochimie 93, 175-186. PUBMED
Singh A.K., McGoldrick, L.L., and Sobolevsky, A.I.(2018) Expression, purification and crystallization of the transient receptor potential channel TRPV6. Methods in Molecular Biology. PUBMED
*Authors contributed equally
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