Nanotechnology is the study of the controlling of matter on an atomic and molecular scale. Generally nanotechnology deals with structures of the size 100 nanometers or smaller in at least one dimension, structural features are intermediate in between isolated atoms and bulk materials, in the range of 10-9 to 10-10 meters, the objects often display physical attributes substantially different from those displayed by earlier atoms or bulk materials.

There are many methods for Nanoparticles synthesis but chemical reduction method is frequently used and characterized using NMR, XRD and FT-IR. Fluorescent molecules enable scientists to detect particular subunit of biomolecular complex, such as live cells with exquisite sensitivity and selectivity fluorescent.

Photobleaching is a photochemical reduction of a dye or a fluorophore signal. Photobleaching creates complications in the observation of fluorescent molecules, since they will be eventually destroyed by light exposure necessary to stimulate them into fluorescing. This phenomenon is problematic in Time-lapse microscopy or fluorescent microscopy. So, Nanoparticles are new subject for bioimaging rather than fluorescent molecules because of its long fluorescent signal stability. These concerns have led to a debate among advocacy groups and governments on whether special regulation of nanotechnology is warranted.

During MSc project work, nanoparticles were successfully synthesized, characterized and evaluated their role in bioimaging.