Our study highlights the decline of UPF1, a key component of nonsense-mediated mRNA decay, during cellular senescence and its impact on NMD efficiency, leading to the accumulation of aberrant mRNAs. 

Extracellular vesicles (EVs) mediate cell-to-cell communication by transferring cargo molecules that influence gene expression and signaling in recipient cells. Our study reveals that UPF2 regulates EV biogenesis by promoting CD81 expression, affecting EV composition and uptake efficiency without altering EV size or quantity.