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The Applied Biosystems 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. This third generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.

Intuitive Software to Run Pre-optimized Protocols

The Applied Biosystems 7500 Fast Dx Real-Time PCR Instrument Sequence Detection Software Security, Auditing, and E-Signature module offers the flexibility to develop assays and also run pre-optimized protocols for users operating in a secure environment.

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USA: For in vitro Diagnostic Use. The 7500 Fast Dx Real-Time PCR instrument is 510(k)-cleared (K082562). The customer is responsible for any validation of assays and compliance with any regulatory requirements that pertain to their procedures and instrument use.

The Applied Biosystems 7500 Fast Dx Real-Time PCR Instrument is an affordable solution for budget-conscious labs. The four color detection system gives users the flexibility to run a variety of applications including SNP genotyping, gene expression, plus/minus assays, pathogen quantitation and more. The instrument is equipped with a CCD camera for precision optics and comes with advanced multi-componenting algorithms for accurate and reproducible results.

The ABI 7500 Real-Time PCR system is an affordable solution for budget-conscious labs. The four color detection system gives users the flexibility to run a variety of applications including SNP genotyping, gene expression, plus/minus assays, pathogen quantitation and more. The instrument is equipped with a CCD camera for precision optics and comes with advanced multi-componenting algorithms for accurate and reproducible results.

The Applied Biosystems 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes. The specially designed Peltier-based 7500 Fast block ensures thermal uniformity at top speeds. Faster ramp rates and novel well design enable rapid results without compromising extension times or assay quality.

If you are interested in the Applied Biosystems 7500 Real Time PCR System be sure to check out our other available PCR and Thermal Cyclers by Applied Biosystems. Request a quote to receive more information on this Applied Biosystems 7500 Real Time PCR System. Have a Applied Biosystems 7500 Real Time PCR System to sell? Fill out this Sell Equipment form.

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Classic hereditary hemochromatosis is an autosomal recessive disorder characterized by iron overload and sequence variants in the HFE gene. The HFE gene is located at 6p21.3 and contains 2 common single nucleotide polymorphisms (SNPs) C282Y and H63D, which are routinely tested for in the molecular diagnostics laboratory. In this study, we used DNA samples from 59 patients in which clinicians wanted to confirm or rule-out hereditary hemochromatosis that had been previously tested for the HFE SNPs using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay and the ABI 7700 real time PCR assay with a MGB Eclipse ASR Probe system. The new assay used TAQman SNP Genotyping Assays, which were performed on the ABI 7500 FAST real time PCR platform. Allelic discrimination was determined during a postamplification plate read. Of the 59 samples genotyped, 7 were homozygous for C282Y, 6 were heterozygous for C282Y, 9 were homozygous for H63D, 10 were heterozygous for H63D, 6 were compound heterozygotes, and 20 were wild type. With the exception of one sample that was indeterminate by the TAQman SNP Genotyping Assay, all others showed 100% concordance between the 3 assays. The one indeterminate sample was heterozygous for C282Y by the PCR-RFLP and ABI 7700 real time PCR assays, but there was an insufficient quantity of DNA to perform the TAQman SNP Genotyping Assay. Our study suggests that the ABI 7500 FAST TAQman SNP Genotyping Assay is comparable with the PCR-RFLP and ABI 7700 real time PCR methods in detecting and characterizing these 2 HFE SNPs. Improved software and thermocycling capabilities have resulted in a very robust TAQman assay with the advantage of a much improved turn-around-time and throughput.

2.0 Scope

This document is intended for laboratory personnel responsible for CLIA regulated processing and testing of clinical specimens for C. auris, using the ABI 7500 Fast Real-Time PCR System.

7.0 Safety Precautions

7.1 Clinical Candida auris specimens (Swabs/Sponge) or isolates have the potential to transmit infectious diseases. Wear protective gloves, lab coat, and eyewear when handling samples.

7.2 All BSL-2 practices, safety equipment, and facility design must follow the requirements described in the BMBL and the Biosafety Manual.

7.3 All biohazardous liquid and solid waste and sharps are handled per agency-specific waste handling policies.

7.4 Laboratory personnel should complete appropriate safety training and Candida-specific laboratory training.

7.6 Review safety section of equipment manuals prior to performing procedure.

7.7 Follow the instrument specific guidance from Roche for decontamination of the MagnaPure 96, or equivalent extraction system.

7.8 Review all SDS for chemicals used in this procedure.

7.9 Follow institutional guidelines for Spills and Incident Reporting.

7.10 Work with unidentified and pathogenic yeast must be performed in an operational Class II A2 BSC.

7.11 BSCs should be decontaminated before and after use with 10% Bleach for a contact time of 10 minutes.

7.12 10% Bleach must be prepared daily for effective decontamination of C. auris or a bleach stabilized solution like Oxivir TB should be used according to the manufactures recommendations

Reagent Handling and Storage

8.7 Store all primers and probes at 2-8C until re-hydrated for use; store all control materials at 20C.

8.8 Always check the expiration date prior to use. Do not use expired reagents.

8.9 Protect fluorogenic probes from light.

8.10 Primers, probes (including aliquots), and enzyme master mix must be thawed and kept on ice or cold block at all times during preparation and use.

8.11 Control DNA must be thawed and kept on ice at all times during preparation and use.

8.12 Clean and decontaminate all work surfaces, BSC, pipettes, and other equipment with a proper C. auris-approved decontamination solution such as 10% bleach solution before and after use.

8.13 Use separate and dedicated equipment (e.g., BSC, pipettes) and supplies (e.g., microcentrifuge tubes, pipette tips) for master mix preparations, setup of Optical 96-Well reaction plate, and addition of DNA template in the wells and decontaminate with UV light and/or ELIMINase to prevent DNA contamination after work is completed.

8.14 Reagents, master mix, and DNA should be maintained on cold block during preparation and/or use to ensure stability

8.37 Determine the number of reactions being performed per assay. Be sure to account for all positive and negative controls, and triplicates. Note: If the number of samples and controls is 14 make enough for two extra reactions. This will account for pipette error.

8.38 Calculate the amount of each reagent needed to make up each master mix using the Master Mix Preparation Worksheet, Attachment # 1.

8.39 Combine all reagents listed on Attachment # 1 in the corresponding labeled tube using an appropriate size pipette.

8.40 Pulse vortex master mix 3 times for 5 seconds to homogenize.

8.41 After all master mixes have been prepared, prepare the NTC reactions.

DNA Template Addition

8.42 Working in a BSC, gently vortex extracted nucleic acid samples and positive control tubes and place on ice or in a cold rack.

8.43 Set up reaction plate on ice or in a cooler rack.

8.44 Refer to Master Mix Preparation Worksheet, Attachment #1 and add appropriate volume of Bicoid DNA (1L/Reaction) in appropriate master mix tubes.

8.45 Pulse vortex master mix 3 times for 5 seconds to homogenize.

Now the easy-to-use StepOne software is available for both the 7500 and 7500 Fast systems with the 7500 Software v2.x upgrade. The 7500 Software v2.x incorporates your favorite StepOne Software features, such as a variety of plate setup wizards, standard curve dilution and master mix recipe calculators, QC flags, data filters, and email notification when a run is finished. The 7500 Software v2.x also includes an enhanced Gene Expression Study package and has a variety of new melting curve protocol options, including multiple peak detection, step and hold temperature control, and customizable ramp rates. 2351a5e196