Zebrafish workshop
Facilitated by Dr Tarryn Lee Botha-
Institute for Nanotechnology and Water Sustainability
Assisted by Ms Makolobe Mabotja-
National Aquatic Bioassay Facility, North-West University
VENUE: National Aquatic
Bioassay Facility in the basement of
building G23
As this is a hands-on training workshop the following schedule is a guideline
07:00 Registration and morning tea/coffee
08:00 Zebrafish breeding (Demonstration)
09:00 Introduction to Zebrafish husbandry (Lecture)
10:00 Tea break
10:30 Juvenile stages development, sorting and handling (Hands-on)
11:30 Tank setup and Zebtec system (Hands-on)
12:00 System failures and emergency procedures
(International invited speaker- virtual lecture)
13:00 Lunch break
14:00 Facility inspections (Hands-on)
14:30 Health monitoring and necropsy (Hands-on)
15:00 Infectious diseases in zebrafish (Invited international
speaker- virtual lecture)
16:00 Reflections and closure
18:00 Cocktail function @ NWU Sport Village,
36 Loop St, Noordbrug, Potchefstroom
SAALAS conference day 01 (Venue - The Roots)
Transport from NWU Sport Village leaves at 07:00 for The Roots (Prebooking required)
7:00 – 8:00 Registration and morning tea/coffee
8:00 – 8:30 Welcome note
SAALAS President, Dr Tamsyn Fourie
8:30 – 9:00 Address by the SAVC President Dr Alfred Kgasi (Online)
9:00 – 10:00 Keynote address 'Global perspectives on laboratory animal science, welfare and ethics' – Dr Barney Reed
10:00 – 10:30 Tea/coffee break
Session 1: Zebrafish
Session chair: Dr Tarryn Botha
10:30 – 10:50 Zebrafish, environmental, microbiological monitoring – Dr Gianpaolo Milite (Online)
10:51 – 11:10 Environmental Enrichment for laboratory zebrafish - a review of the evidence - Dr Chloe Stevens (Online)
Session 2: Animal Ethics
Session chair: Dr Dave Lewis
11:11 – 11:30 Transitioning animal ethics training to the online environment – pros and cons: Prof Tiaan Brink & Prof Ché Weldon
11:31 – 11:50 Transitioning animal ethics training to the online environment – pros and cons: Prof Tiaan Brink & Prof Ché Weldon
11:51 – 12:10 Common errors in animal ethics applications - Professor Md. Shahidul Islam
Session 3: Animal facility and disaster management
Session chair: Dr Kim Jardine
12:11 – 12:40 Disaster Plan for Animal Care and Use - A Case Study – Dr Nico Minnaar
12:41 – 13:00 Environmental Monitoring: Ultrasound Noises - Mr, Jabu, Magagula
13:00 – 14:00 Lunch break
Session 4: Education and training. Session chair: Dr Bert Mohr
14:00 – 14:20 PAN-LASE: Building human capacity in research animal sciences and ethics across Africa – Dr Dave Lewis
14:21 – 14:40 Hybrid learning: lessons learnt from presenting a Laboratory Animal Science course during the COVID-19 pandemic at the University of Cape Town Research Animal Facility - Mrs Kerith Coulson
14:41 – 15:00 Collaboration within the Para-veterinary professionals in South Africa – Mr Justice Malumedzha
15:01 – 15:20 Short Learning Programme for Laboratory Animal Technologists – Dr Johan Oosthuizen (Online)
15:21 – 15:40 Tea/coffee break
15:41 – 16:00 Poster presentations
Mice on ice: cryo-banking mouse germplasm for the future - Mrs Kerith Coulson
The implication of cage changing frequency on welfare of mice and their environment - Mr Rembuluwani Mulaudzi
Volatile anaesthetic delivery (VAD) system for fish- Dr Richard Mavunganidze.
The importance of environmental enrichment for laboratory animals used for scientific and teaching purposes - Mrs. Fiona, Mulaudzi
Maintaining microbiological isolators as a means to protect nucleus mouse colonies against disasters - Mr. Carl Jaftha
16:00 – 17:00 SAALAS AGM and Exco elections
Transport from Roots to NWU leaves at 17:00 (prebooking required)
Transport from NWU to the Roots leaves at 18:00 (prebooking required)
18:00 Gala dinner & Exco announcement: Venue: The Roots
Transport from Roots to NWU leaves at 21:00
SAALAS conference day 02 Venue: The Roots
Transport from NWU Sport Village leaves at 07:00 for The Roots
6:00 – 7:00 5-KM fun run/walk at the Potchefstroom Park Run – Mr Jabu Magagula
7:00 – 8:00 Registration and morning tea/coffee
Session 6: Animal welfare
Session chair: Mr Barney Reed
8:00 – 8:20 A comparison of welfare indicators of group-housed rats in semi-naturalistic and standard cages - Dr Richard Mavunganidze
8:21 – 8:40 The development and optimization of a laboratory-based test for the screening of tuberculosis in non-human primates. - Miss Asisipho Mzukwa. (Online)
8:41 – 9:00 A case of Primary splenic histiocytic sarcoma (HS) in a Vervet monkey (Chlorocebus pygerythrus). - Dr Richard Mavunganidze
Session 7: Scientific session
Session chair: Prof Joshua Olowoyo
9:01 – 9:15 Effect of amaranth leaf meal inclusion level on growth performance, blood profiles and gut morphology of indigenous Boschveld chickens - Tlou Grace Manyelo
9:16 – 9:30 The impact of dietary salt on the development of hypertension and gut microbiome dysbiosis in captive-bred vervet monkeys (Chlorocebus aethiops) - Dr. Zandisiwe Emilia Magwebu
9:31 – 9:45 The association between genetic variants and gene expression in RAAS genes using captive-bred Vervet monkeys (Chlorocebus aethiops) - Miss Sanele Khoza (Online)
9:46 – 10:00 The genetic screening of PCSK9 gene using a non-human primate model of atherosclerosis - Mr. Thobile Ngqaneka (Online)
10:00 – 10:30 Tea/Coffee break
10:30 – 10:45 Boophone disticha attenuates effects of swimming stress on behaviour and neuroblast differentiation in Balb/c mouse hippocampus. - Mr Nkosiphendule Xhakaza
10:46 – 11:00 The Syrian Hamster as an animal model for SARS-CoV-2 – Busisiwe Mogodi
Session 8: Harms-benefit Assessment and the 3R’s
Session chair: Dr Tamsyn Fourie
11:01- 11:20 Implementation of the 4R’s (Replacement, Reduction, Refinement, and Responsibility) in laboratory animal science at the University of Cape Town, South Africa - Sr Janet McCallum
11:21 – 11:40 See what you have been missing: what locomotor activity can teach us in terms of refinement, reduction and replicability ‘round the CLOCK (24/7) animal studies - Dr. Stefano Gaburro
(Online)
11:41 – 12:00 Animal Research: Time to Talk! - Kirk Leech (Online)
12:00 – 12:40 'Culture of care' story: Do we care? Dr Barney Reed
Closure of SAALAS Conference
12:41 – 13:00 Closing remarks Dr Tamsyn Fourie
13:00 – 14:00 Lunch break
14:00 – 14:30 Welcome note: Education & training workshop
14:30 – 16:00 Focus on Animal Caretakers
16:00 – 16:30: Tea break
16:30 – 17:00 Parallel sessions
Animal Caretakers (panel review): room 1
Vets & Para-vets (brainstorming groups): room 2
17:00 – 17:30 Feedback by Animal Caretakers (knowledge & competencies)
18:00 - Evening fun activity & dinner (sponsored)
Venue: The Roots
07:00 – 08:00 Registration and morning tea/coffee
08:00 – 09:40 Parallel sessions
Animal Caretakers (panel review): room 1
Vets & Para-vets (brainstorming groups): room 2
9:40 – 10:00 Feedback by Animal Caretakers – Draft Learning Outcomes Framework
10:00 – 10:30 Tea/Coffee break
10:30 – 12:30 Feedback by Vets & Para-vets: Collation of Life-Long Learning Roadmap
12:30 – 12:50 Way forward and plans for implementation
12:50 – 13:00 Closure of E&T workshop
13:00 – 14:00 Lunch break
14:00 Shuttles to airport (for people who pre-booked online only)