Technical Protocols - Student Collection

Videos collected by SCRM 525 Students:

Assessment of protein expression by IF

Immunofluorescence (Direct and Indirect)

Immunoflourescence

Indirect Immunoflourescence on frozen section of mouse mammary gland

Confocal Fluorescence Microscopy: A Technique to Determine the Localization of Proteins in Mouse Fibroblasts

Heterogeneity of protein expression in tumors

Isolation culture and characterization of differentiation human muscle stem cells

Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods

Immunoflourescence assay (IFA) – Immunology Virtual Lab | PraxiLabs

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1⁺ Tim-3⁺ CD8⁺ T Cells

Explant Tutorial for Culturing Stem Cells

Immunoflourescence microscopy staining paraffin

Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

Isolating and culturing primary stem cells from humans and mice

How to prepare a single cell suspension from mouse spleen

Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure

Isolation of MSCs from mouse compact bone

Pluripotent Stem cell culture in NutriStem® XF/FF

Isolation, Culture, and Transplantation of Muscle Satellite Cells

Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells

How to Isolate Cells in 3 Easy Steps using MACS MicroBeads

Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord

Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue

Isolation, fixation IF imaging of mouse adrenal

One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice

Primary Cell Culture: Protocols & Guidance

Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates

Allen Cell Methods: Single cell passaging human iPS cells

Derivation of Human Embryonic Stem Cells by Immunosurgery

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Techniques for assessing chromatin structure

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-seq

Mapping genome wide accessible chromatin primary human t lymphocytes

ATAC-seq Assay with Low Mitochondrial DNA Contamination from Primary Human CD4+ T Lymphocytes

ChIP-Seq/ATAC_seq Analysis and Integration with Gene Expression Data

Chromatin Immunoprecipitation

Visual Protocol

Lineage analysis and barcode tracking

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM)

Proteomics sample preparation

Lineage Tracing by Cre-LoxP System

Introduction to proteomics

QMUL Science Alive: Protein expression and purification

Introduction to Mass Spectrometry

Proteomics Day 1

Organoid production and assessment

How to grow cerebral organoids

Generation of Tumor Organoids from Genetically Engineered Mouse Models of Prostate Cancer

Mouse Intestinal Organoid Culture with IntestiCult™ Organoid Growth Medium

Handling and assessment of human primary prostate organoid culture

Kidney Organoids: Flow-Enhanced Vascularization and maturation In Vitro

Generation and application of 3D-organoid cell culture models using human iPSCs

Mass Cytometry Introduction