Instrument: Shimadzu Nexera XR (ultra) high pressure liquid chromatography comes equipped with three modes of detection: the SPD-M20A and either the RF 20A or Sedex 90 ELSD detector. The SPD-M20A is a photodiode array detector that has deuterium (D2) and tungsten lamps, which can be used to scan wavelengths from 190 to 800 nm. From this detector, samples can either go to the RF 20A or the ELSD detector. The RF and ELSD detectors are destructive, so no other detection can occur after those processes. The RF20A is a fluorescence detector and the ELSD is a light scattering detector. The RF20A uses a Xenon lamp, which emits at wavelengths between 200 and 650 nm. The ELSD detector measures light scattered from a 405 nm laser. The Nexera XR HPLC can use up to 6 columns, 8 mobile phases, and 10 gradient settings for 96 possible combinations. This reduces preparation time and makes analyte separation easier and more reliable.

HPLC is generally used for the quantification of organic compounds and biomolecules. Samples should be filtered with a 0.22 µm filter before analysis and reference standards are required for a quantitative analysis. First time users need to require Shimadzu training and search the literature for a method before scheduling their appointment.

Our HPLC is made up of the following units: DGU20A, LC30AD, SIL-30AC, CTO-20A, CBM-20A, SPD, M20A, RF 20A, Sedex 90 ELSD.

Location: Fascitelli Center for Advanced Engineering, Suite 067, 2 East Alumni Avenue, Kingston, RI 02881.

Instrument Reservation: Contact coeanalytical@etal.uri.edu for instrument reservation.

Columns can be found at the following link: https://www.shimadzu.com/an/hplc/column/index.html

Vials: https://www.ssi.shimadzu.com/products/consumables/vials.html