Zohar Sachs Full Image

Sachs, et al; Blood 2014

Image courtesy of Zohar Sachs; showing SPADE trees of untreated (left) and treated (right) cell populations. Circle sizes represent the size of the cell populations matching a specific protein profile, colors indicate signal intensity for the evaluated protein marker for that rendering. This figure is rendered for two different markers. This SPADE analysis was performed on data generated at Stanford University in collaboration with Garry Nolan's lab. CyTOF analysis allowed us to identify unique signaling characteristics of the stem cell. The Mac-1 population harbors the leukemia stem cell. We found that β-catenin is localized to this stem cell compartment (the branch is encircled and labeled Mac-1 Low. Loss of NRAS (G12V) leads to the loss of β-catenin-rich cells. β-catenin is critical for myeloid leukemia self-renewal.