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Litesizer 500 - Particle Size and Zeta Potential Analyzer
Characterization of particles in nanometer to micrometer ranges: 0.3 nm to 10 µm
Particle size measurements through dynamic light scattering at three different measurement angles (15°, 90°, 175°)
Zeta potential determination using patented cmPALS
Molecular mass measurements: 300 Da to 20 MDa
Refractive index measurements: 1.28 a 1.50
Minimum concentration: 0.1 mg/mL (proteína) ou 0.1 ppm
Location: UENF/CBB/LQFPP - Building P5 (CBB/CCT) - Room #219
Contact: Prof. Luís Basso - luisbasso @ uenf.br
Funding: UENF
Scheduling (subject to approval): click here
Electron Paramagnetic Resonance (EPR) Spectrometer
Bruker Elexsys E500
Mode: Continuous wave
Operating frequencies: X-band (9.5 GHz) and Q-band (35 GHz).
Location: UENF/CCT/LCFIS - Anexx of Building P3 (CCT)
Contact: Prof. Luís Basso - luisbasso @ uenf.br
Funding: FAPERJ
Scheduling (subject to approval): click here
Nuclear Magnetic Resonance (NMR) Spectrometer
Bruker Avance III HD 500MHz NMR, ASCEND ULH
11.7 Tesla (500 MHz for hydrogen frequency), equipped with a 5 mm BFO probe (smart probe with ATMA®) for liquid-state sample acquisition and a 4 mm CP/MAS probe for solid-state sampling.
Location: UENF/CCT/LCQUI - Anexx of Building P3 (CCT)
Funding: FAPERJ
Fluorescence Spectrofluorometer
Varian/Agilent Cary Eclipse Model
Steady-state fluorescence
Location: UENF/CCT/LCQUI - Building P3 (CCT), Laboratory 103
Contact: Prof. Sergio Luis Cardoso - cardoso @ uenf.br
Funding: FAPERJ
Manuals:
Varian/Agilent Eclipse - Folder
Circular Dichroism Spectropolarimeter
Jasco J-T815 Model
Circular dichroism measurements in the UV/Vis region
Light source: 150 W Xe arc lamp
Wavelength range: 190 to 900 nm
Location: UENF/CBB/LQFPP - Building P5 - Laboratory 219
Contact: Prof. Ana Eliza Zeraik - anazeraik@uenf.br
Funding: FAPERJ
FT-IR Spectrophotometer
Perkin Elmer Model Spectrum Two
Detector: LiTaO3 - MIR (RSR 9.2300:1)
Scanning range: 8,300 – 350 cm-1 (at 0.5 cm-1 resolution)
Accessories: Horizontal Attenuated Total Reflectance (HATR) Accessory
Diffuse Reflectance Accessory
Location: UENF/CCT/LCFIS - Building P3 - Annex
Contact: Prof. Luís Basso - luisbasso@uenf.br
Funding: FAPERJ
Manuals:
FT-IR - Perkin Elmer Spectrum Two - Video
FT-IR - Perkin Elmer - Spectrum Two - User Manual
FT-IR - Perkin Elmer - Spectrum Two - HATR Manual
FT-IR Perkim Elmer - Spectrum Two - Diffuse Reflectance Manual
Nano ITC (Isothermal Titration Calorimeter)
Low Volume Model from TA Instruments
Sample cell volume: 190 µL
Injection syringe volume: 50 µL
Minimum injection volume: 0.06 µL
Temperature range: 2 °C to 80 °C
Location: UENF/CBB/LQFPP - Building P5 - Laboratory 219
Contact: Prof. Ana Eliza Zeraik - anazeraik@uenf.br
Funding: UENF
Microvolume UV-Vis Spectrophotometer - NanoDrop
Model NanoDrop™ One/One
Full spectrum with wavelength measurement range from 190 nm to 850 nm
Sample volume: 1 to 2 µL
Concentration range: 2 - 27500 ng/µL dsDNA and 0.06 to 820 mg/mL BSA
Location: UENF/CBB/LQFPP - Building P5 - Laboratory 219
Contact:
Prof. Ana Eliza Zeraik - anazeraik@uenf.br
Prof. Luis G. M. Basso - luisbasso@uenf.br
Funding: FAPERJ
UV-Visible Spectrophotometer
Varian/Agilent Model Cary 50 Bio
Location: UENF/CCT/LCQUI - Building P3 - Laboratory 103
Contact: Prof. Sergio Luis Cardoso - cardoso@uenf.br
Funding: FAPERJ
Manuals:
UV-VIS-Varian-Cary-50-Bio-Folder
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