OUR DESIGN

Finalized Design

Figure 1: Image carousel demonstrating the finalized team design. A CAD design of the model and flow diagram have been included. The finalized microscope contains a custom-built slide holder (seen in the last image), a 3-axis motorized stepper motor stage, Whitefield illumination (phone), a 20x objective, and a 12-megapixel camera. This design was built for simplicity and cost-effectiveness, with a simple scanning function and a total cost of around $3.5k. 

Figure 2: Google sheet containing all components considered for purchasing. In the end, only the first three items were outsourced as they are the generally the most expensive components. The stage set-up cost around $2.2k before tax while the camera came out to $250. After tax and import fees however, the total cost of outsourced parts was approximately $3k. 

Alternative Designs

Figure 3: Image carousel of alternative designs considered by the team. The first design is for a cheap non-microfluidic LED microscope. This microscope contained two columnated LEDs, a 3-axis stepper motor stage, a 60x objective, as well as a dichroic mirror. The second design is the original prototype, an industry-level non-microfluidic microscope. The main feature of this microscope was its four laser engines and fiber optics for illumination and localization.

Poster Presentation of the Project: Condensed and simplified version of the Project

Applicable Standards

 ISO 19012-1:2013, ASTM E2719-09 (2022), ISO 19056-3:2022, and ISO/ASTM 52900:202. ([1],[2],[3],[4])

These standards address quality control in aspects such as lighting, and flatness of the field, and define terms for manufacturing practices such as 3D printing.

ISO 9345:2019, ISO 10935:2009 ([5], [6])

Standards for adapters and connections including lens mounts, screw threadings, and objective adapters. 

As this is not a medical device to be used on or in the body, standards for sterility in packaging, biocompatibility, and clinical testing are not relevant. Overall, the device is extremely safe, with very few safety considerations other than wiring and the stage. 

References

1. Microscopes — Designation of microscope objectives — Part 1: Flatness of field/Plan, ISO 19012-1:2013, International Organization for Standardization, 2013.

2. Standard Guide for Fluorescence—Instrument Calibration and Qualification, ASTM E2719-09(2022), American Society for Testing and Materials, 2022.

3. Microscopes — Definition and measurement of illumination properties, ISO 19056-3:2022, 2022.

4. Additive manufacturing — General principles — Fundamentals and vocabulary, ISO/ASTM 52900:2021, 2021.

5. Microscopes — Interfacing dimensions for imaging components, ISO 9345:2019, 2019.

6. Microscopes — Interfacing connection type C, ISO 10935:2009, 2009.

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