Embedded Files
Sample Preparation
Sample Preparation
Samples prepared via xenografted human tumor subtype tissue into mouse models. The xenograft model was used due to being advantageous for 3D structure development for the tumors.
D2O tracing was used to improve spectral imaging (C-D bonds).
Once the tumors were sufficiently developed they were excised and placed on slides for imaging.
Sample Imaging
Sample Imaging
Spontaneous Raman Spectroscopy + LabSpec6 Software.
A. Focusing on a random extracellular region of interest on the breast cancer tissue slide.
B. Adjusting the focal plane to maximize the signal-to-noise ratio.
C. Acquiring the sample spectra at the optimized focal plane.
D. Focusing on the blank area on the slide at the same focal plane and acquiring a background spectra. The software-installed algorithm will subtract the background spectra from the sample spectra.
Data Processing
Data Processing
Noise Subtraction
Noise Subtraction
Noise subtraction with respect to the cell silencing region (2500-2600) eliminates impact of light scattering of the slide and Raman scattering of PBS.
Normalization
Normalization
Normalization of the whole spectrum with respect to the 2930 peak enables more intuitive comparsion between different measurement trials.
Averaging
Averaging
Averaging all processed Raman spectra from the same breast cancer subtype tissue gives a representative information on the metabolic activities.
Page developed by Hester Deng
Page updated
Report abuse