Introduction to CRISPR
Introduction to CRISPR
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The introduction of CRISPR/Cas9 technology has revolutionized the field of functional genomics by providing a novel tool for interrogating gene function. CRISPR/Cas9 is a deoxyribonuclease that can be specifically targeted to genomic regions via a guide RNA (gRNA). Targeting of Cas9 to a region results in a blunt double-stranded DNA break that engages the cellular nonhomologous end joining (NHEJ) DNA repair pathway. This promotes imprecise repair and produces small deletions in the repaired sequence. These small deletions then result in a frameshift that disrupts the ORF within coding genes and disrupts protein synthesis.
The introduction of CRISPR/Cas9 technology has revolutionized the field of functional genomics by providing a novel tool for interrogating gene function. CRISPR/Cas9 is a deoxyribonuclease that can be specifically targeted to genomic regions via a guide RNA (gRNA). Targeting of Cas9 to a region results in a blunt double-stranded DNA break that engages the cellular nonhomologous end joining (NHEJ) DNA repair pathway. This promotes imprecise repair and produces small deletions in the repaired sequence. These small deletions then result in a frameshift that disrupts the ORF within coding genes and disrupts protein synthesis.
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