CRISPR Libraries:

Human

Whole-genome coding CRISPR editing:

targets all refseq annotated coding genes and microRNAs
12 guides per gene
5000 negative control guides
~270k total guides

Whole-genome coding CRISPR interference (CRISPRi):

targets all refseq annotated coding tanscripts and microRNAs
12 guides per gene
5000 negative control guides
~270k total guides

Whole-genome lncRNA CRISPR interference (CRISPRi):

targets all refseq annotated lncRNAs
3000 positive controls genes (go-term: apoptosis, immune, CD antigens)
12 guides per gene
3000 negative control guides
~94k total guides

Macrophage-specific lncRNA CRISPR interference (CRISPRi):

targets all expressed lncRNAs (THP1)
55 positive controls
10 guides per gene
~800 negative control guides
~25k total guides

Libraries published here

Mouse

Whole-genome coding CRISPR editing:

targets all refseq annotated coding genes and microRNAs
12 guides per gene
5000 negative control guides
~270k total guides

Whole-genome coding CRISPR interference (CRISPRi):

targets all refseq annotated coding tanscripts and microRNAs
12 guides per gene
5000 negative control guides
~270k total guides

Macrophage-specific lncRNA CRISPR interference (CRISPRi):

targets all expressed lncRNAs (THP1)
55 positive controls
10 guides per gene
~800 negative control guides
~25k total guides

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