Features

• • Resonant Scanner for high-speed imaging at up to 30 fps at 2048 x 512 pixels 

    • • Up to 2048 x 2048 pixels for 2K

  • Galvano Scanner for high-resolution imaging at up to 8192 x 8192 pixels

• 6 laser lines (405/445/488/514/561/640)

• Objectives: 

  • • CFI60 Plan Apochromat Lambda D 4x Objective Lens, N.A. 0.2, W.D. 20.0mm, F.O.V. 25mm

    • CFI60 Plan Apochromat Lambda D 20x Objective Lens, N.A. 0.80, W.D. 0.80mm, F.O.V. 25mm, DIC, Spring Loaded

    • CFI60 Apochromat Lambda S LWD 40x Water Immersion Objective Lens, N.A. 1.15, W.D. 0.59-0.61mm, F.O.V. 22mm, DIC, Correction Collar 0.15-0.19mm

    • CFI60 Plan Apochromat Lambda D 60x Oil Immersion Objective Lens, N.A. 1.42, W.D. 0.15mm, F.O.V. 25mm, DIC, Spring Loaded

• 3 GaAsP and 1 Multi-alkali PMT detectors (2 spectral and 2 filter-based)

• AX Transmitted Detector (for D.I.C. imaging)

• NSPARC Single Photon Activating Detector with Super Resolution

• Tokai Hit Stage-Top Environmental Chamber with Digital Gas Mixer

• Nikon DS-Ri2 Color CMOS camera (4908x3264 pixel, full pixel)

• Piezoelectric Z

• Nikon Ti2-E Motorized Inverted Microscope with Perfect Focus System and 25 mm field of view

Features

• 4 laser lines (405/488/555/639)

• Objectives: 

  • • Plan NeoFluar 10x Objective Lens, N.A. 0.3, Ph1

    • Plan-Apochromat 20x Objective Lens, N.A. 0.80

    • C-Apochromat 40x Water Immersion Objective Lens, N.A. 1.2, Correction Collar

    • Plan Apochromat 63x Oil Immersion Objective Lens, N.A. 1.4, DIC

    • Plan Apochromat 100x Oil Immersion Objective Lens, N.A. 1.46, DIC, (UV) VIS-IR

• 2 Multi-alkali PMT detectors with spectral tuning

  • Resolution: 2048 x 2048

  • Up to 5 fps of 512 x 512 pixels

• Transmitted Detector for D.I.C. imaging

• Fully motorized Axio Observer.Z1 with 18 mm field diagonal

Features

• D-LEDI Light Engine with four LEDs (385 nm, 475 nm, 550 nm and 621 nm)

• LED Transmitted Light 

• Prior Scientific Proscan XY Motorized Stage with Encoded Focus Drive 

• Objectives: 

  • • CFI PLAN FLUOR 4X NA 0.13 WD 17.2MM CH

    • CFI PLAN FLUOR DLL 10X NA 0.3 WD 16MM CH 

    • CFI PLAN FLUOR DLL 20X NA 0.5 WD 2.1MM C 

    • Plan Apochromat 60x Air Objective Lens, N.A. 0.8

• DS-QI2 MONOCHROME CMOS CAMERA 

  • Resolution: 4908x3264 full pixel (max 6fps) / 3x3 pixel averaging 1636x1088 (max 45fps)

  • 77% quantum efficiency

• Basler MED ace 2.3 MP 41 color CMOS camera

  • 1920 x 1200 at 41 fps

• Stereologer Software (SRC Biosciences) for unbiased stereology

• NIS elements BR software

• Eclipse Ti2A microscope body with 25 mm FOV

HP Z4 PC Workstation

• • Xeon 4.2G processor-6 core

  • 128GB RAM

  • NVIDIA RTX A4000 16GB GPU

  • 1TB M.2 DDS OS Drive; 12TB SATAdatadrive

• NIS-Elements Advanced Research Software 

  • General Analysis modules (GA2 and GA3)

  • Extended Depth of Focus (EDF) module

  •  LIM's 2D/3D Deconvolution Suite

    • Includes batch de-convolution

  • Ratio Module: Ratio View, Live Ratio Graphing & Measurement, Calcium & New Titration Calibration, FRET: Capture and Analysis

• XP Pen Artist 24 Display Tablet