Our expected graph in Figure 4 would show that reduction in the expression of PA14_54520 would lead to a decrease in bacterial growth in artificial urine media. This would be expected if reduction in OdpH expression would lead to inefficient uptake of cis-aconitate as a carbon energy source
.

These expected results and trends would support our hypothesis that the PA14_54520 utilizes the OpdH protein to transport cis-aconitate as an energy source needed for survival.

Our alternate results in Figure 5 display unregulated bacterial growth in both the parental and knockdown strains. There could be no significant difference in the bacterial growth of PA14_54520 knockdown strain compared to the parental strain. A rationale for the alternative results would be that the bacteria compensates for the reduced expression of the gene by using other protein channels to uptake necessary metabolizing compounds essential for survival.

Both graphs depict how the knockdown of the PA14_54520 gene could affect the ability of the bacteria to grow in a nutrient limited environment (artificial urine media).