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Sir Alexander Fleming, a Scottish physician and microbiologist, returned from his holiday on 3 September 1928 to continue his project at St. Mary's Hospital in London. He noticed a bluish-green mold growing on his petri dish. He extracted it and investigated it further.
It turned out that the mold was inhibiting the growth of Staphylococcus bacteria. He examined this mold further to realize it can easily kill off any bacteria. Back in the 1920s, bacterial infections were a common cause of deaths in the world. Therefore, he accidentally discovered a solution to that problem.
Soon, he kept producing the bacteria-killing mold and figured out ways to improve by mixing it with other chemicals such as corn steep liquor. This mold was called a Penicillium, it produces a juice to inhibit the formation of a bacterium's cell wall. The mold juice disrupts the synthesis of peptidoglycan, a key component of a bacterium's cell wall. Without a proper cell wall, the bacterium becomes vulnerable to internal pressure, causing it to burst and die.
However, bacteria gradually adapted to becoming stronger against Penicillin. Thus, it became easier for bacteria to fight the Penicillium and other components of Penicillin. Nonetheless, with the help of organic and medicinal chemistry, new solutions for Antibiotics were generated and produced. The antibiotic's structure and adaptations were analysed, allowing scientists to modify it by fermenting it to make it pure, increasing its effectiveness. With modern-day technologies in addition up our sleeves, we can easily make new Antibiotics.
Source: The Accident that Created Antimicrobials. How Alexander Fleming's Accident Created a Relevant Medication in Modern Medicine. Retrieved May 25, 2025, from https://www.bbc.co.uk/newsround/articles/c3dv4dj51keo
Journey of Penicillin. Discovery and Development of Penicillin. Retrieved May 25, 2025, from https://www.acs.org/education/whatischemistry/landmarks/flemingpenicillin.html
This antiviral was invented by Gertrude Belle Elion (1918-1999). Elion was an American pharmacologist and biologist who worked alongside George Hitchings (1905-1998), an American medical doctor. Both scientists had a desire to create highly targeted drugs that selectively block the growth and replication of pathogens and cancer cells. They sought to exploit differences in nucleic acid metabolism between normal human cells and these disease-causing organisms, aiming to develop treatments that wouldn't harm healthy cells.
On the left is George Hitching, and on the right is Gertrude Elion
Sources: How Two Scientists Created Antimicrobials That Saved the World. Hitchings and Elion's Story. Retrieved May 27, 2025, from https://www.sciencehistory.org/education/scientific-biographies/george-hitchings-and-gertrude-elion/
A tube of Acyclovir cream
(used to treat chickenpox, shingles, and herpes)
Basically, they applied their knowledge of biochemistry and analytical chemistry. Being a food quality control worker during the Great Depression, Elion was highly experienced in analytical techniques. As a result, they studied nucleic acids, which are fundamental building blocks of DNA and RNA, and developed methods to analyze these bases - these included research on how the RNA and DNA metabolizes the drug without causing harm, as well as the biochemical differences between normal cells and pathogen cells to target specific cellular processes. This required analytical methods to study the structure and function of nucleic acids.