Cryo-electron microscopy (cryo-EM) is a cutting-edge technique employed for the visualisation of biological macromolecules in their near native state.
In single-particle analysis (SPA), the sample of interest is rapidly frozen on a small metal support, which is subsequently imaged in an electron microscope
Sample vitrification (Tiago R.D. Costa et al., 2017)
Frozen cryo-EM grid (Tiago R.D. Costa et al., 2017)
A large number of images are collected from which the biological macromolecules of interest are isolated and averaged
A 3D reconstruction of the macromolecule can be produced by aligning these selected "particles," which represent multiple views of the macromolecule
3D reconstruction in real space (Tiago R.D. Costa et al., 2017)
For more details, please visit the website https://cryoem.iitm.ac.in/
