Name: John Graham White FRS
Born: Prestatyn Wales, 9th August 1943
EDUCATION;
1965-1969 Brunel University, London
B. Tech. (hons.) physics 1969
1970-1974 University of Cambridge
Ph.D. in biology 1975
EMPLOYMENT:
1964-1969 Medical Research Council's
National Institute for Medical Research
Mill Hill, London, U.K.
Awarded undergraduate scholarship in 1965
1969-1993 Medical Research Council's
Laboratory of Molecular Biology
Hills Road, Cambridge, U.K.
Member of tenured staff since 1975.
1993-1996 Visiting Professor of Molecular Biology and Anatomy
Director of the Integrated Microscopy Resource
University of Wisconsin
1525 Linden Drive
Madison, Wisconsin
1996- 2008 Professor of Molecular Biology and Anatomy
University of Wisconsin
2001- 2008 Chair, Laboratory of Molecular Biology
University of Wisconsin
Sabbatical year spent in University of Colorado at Boulder 1978-1979.
Queen's Award for Technology 1991.
Wiliam Bate Hardy Prize of the Cambridge Philosophical Society 1993.
Royal Society Mullard Award 1994
Rank Prize for opto-electronics 1995
Elected Fellow of the Royal Society 2005
Holder of three patents related laser scanning microscopy.
Member of EMBO
PUBLICATIONS
* denotes most significant publications.
1. *Ward, S., Thomson, N., White, J.G. and Brenner, S. (1975). Electron microscopical reconstruction of the anterior sensory anatomy of the nematode Caenorhabditis elegans. J. Comp. Neurol. 160, 313-338.
2. *White, J.G., Southgate, E., Thomson, J.N. and Brenner, S. (1976). The structure of the ventral cord of Caenorhabditis elegans. Phil. Trans. Roy. Soc. Lond. B, 275, 327-348.
3. *Albertson, D., Sulston, J. and White, J.G. (1978). Cell cycling and DNA replication in a mutant blocked in cell division in the nematode Caenorhabditis elegans. J. Dev. Biol. 63, 165-178.
4. *White, J.G., Albertson, D.G. and Anness, M.A.R. (1978). Connectivity changes in a class of motoneurone during the development of a nematode. Nature 271, 764-766.
5. Kimble, J., Sulston, J. and White, J. (1979) Regulative Development in the post-embryonic lineages of Caenorhabditis elegans. INSERM symposium no. 10, pp 59-68, Ed. N. Le Dourin, Elsevier Press.
6. White, J.G. and Horvitz, H.R. (1979). Laser microbeam techniques in biological research. Electro-Optical Systems Design 11, 23-24.
7. Stevens, B.J. and White, J.G. (1979). Computer reconstruction of mitochondria from yeast. In Methods in Enzymology. Vol. 56 (eds. S. Fleischer and S.L. Packer), pp. 718-728, Academic Press, New York.
8. *Sulston, J.E. and White, J.G. (1980). Regulation and cell autonomy during postembryonic development of Caenorhabditis elegans. Dev. Biol. 78, 577-597.
9. *Kimble, J.E. and White, J.G. (1981). On the control of germ cell development in Caenorhabditis elegans. Dev. Biol. 81, 208-219.
10. *White, J.G., Horvitz, H.R. and Sulston, J.E. (1982). Neurone differentiation in cell lineage mutants of Caenorhabditis elegans. Nature 297, 584-589.
11. *Sulston, J.E., Schierenberg, E., White, J.G. and Thomson, J.N. (1983). The embryonic cell lineage of the nematode Caenorhabditis elegans. Dev. Biol. 100, 64-119.
12. *White, J.G., Southgate, E., Thomson, J.N. and Brenner, S. (1983). Factors that determine connectivity in the nervous system of Caenorhabditis elegans. Cold Spring Harbor Ann. Symp. Quant. Biol. Vol. XLVIII.
13. *White, J.G. and Borisy, G.G. (1983). On the mechanisms of cytokinesis in animal cells. J. Theor. Biol. 101, 289-316.
14. *Chalfie, M., Sulston, J.E., White, J.G., Southgate, E., Thomson, J.N. and Brenner, S. (1985). The neural circuit for touch sensitivity in Caenorhabditis elegans. J. Neuroscience 5, 956-964.
15. *Hedgecock, E.M. and White, J.G. (1985). Polyploid tissues in the nematode Caenorhabditis elegans. Dev. Biol. 107, 128-133.
16. White, J.G. (1985). Neuronal connectivity in Caenorhabditis elegans. TINS 8, 277-283.
17. White, J.G. (1985). The astral relaxation theory of cytokinesis revisited. Bio Essays 2, 267-272.
18. White, J.G. and Hyman, A.A. (1986). On the implications of laterally mobile cortical tension elements for cytokinesis. Proceedings of NATO ARW workshop and "The Biomechanisms of Cytokinesis", pp 79-96 (ed. N. Akkar), Plenum Press.
19. *White, J.G., Southgate, E., Thomson, J.N. and Brenner, S. (1986). The structure of the nervous system of Caenorhabditis elegans. Phil. Trans. Roy. Soc. Lond. B. 314, 1-340.
20. *Amos, W.B., White, J.G. and Fordham, M. (1987). The application of confocal imaging to the study of biological structures. J. Appl. Optics, 26, 3239-3243.
21. *Hyman, A.A. and White, J.G. (1987). Determination of cell division axes in the early embryogenesis of C. elegans. J. Cell Biol. 105, 2123-2135.
22. *White, J.G., Amos, W.B. and Fordham, M. (1987). An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy. J. Cell Biol. 105, 41-48.
23. White, J.G. and Amos, W.B. (1987). Confocal microscopy comes of age. Nature 328, 183-184.
24. *Bray, D. and White, J.G. (1988). Cortical flow in animal cells. Science 239, 883-888.
25. White, J.G. (1988). "The Anatomy" in: The Nematode Caenorhabditis elegans. pp 81-122, Ed. W.B. Wood, Cold Spring Harbor Press, New York.
26. Chalfie, M. and White, J.G. (1988). "The Nervous System" in: The Nematode Caenorhabditis elegans. pp 337-391, Ed. W.B. Wood, Cold Spring Harbor Press, New York.
27. Mills, A.D., Blow, J.J., White, J.G., Amos, W.B., Wilcock, D., Laskey, R.A. (1989) Replication occurs at discrete foci spaced throughout nuclei replicating in vitro. J. Cell Sci 94:471-
28. White, J.G. (1990). Laterally mobile cortical tension elements can self-assemble into a contractile ring. Ann. N.Y. Acad. Sci., 582, 50-59.
29. White, J.G., Amos, W.B., Durbin, R. and Fordham, M. (1990). "Development of a confocal imaging system for biological epifluorescence applications" in: Optical Microscopy for Biology, pp1-18, Eds. B. Herman and K. Jacobson, Wiley-Liss, New York.
30. *White, J.G., Southgate, E. and Thomson, J.N. (1991). On the Nature of Undead cells in the Nematode Caenorhabditis elegans. Phil Trans R. Soc. Lond. B. 331, 263-271.
31. *White, J.G., Southgate, E. and Thomson, J.N. (1992). Mutations in the Caenorhabditis elegans unc-4 gene alter the synaptic input to ventral cord motor neurons. Nature 355, 838-841.
32. McIntyre, S.L., Gariga, G., White, J.G., Jacobsen, D. & H.R. Horvitz (1992) Genes Necessary for Directed Axonal Elongation or Fasciculation in C. elegans. Neuron 8, 307-322.
33. *Curley, P.E., Ferguson, A.I., Amos, W.B. & White, J.G. (1992) Application of a femtosecond, self-sustaining, mode-locked, Ti-sapphire laser to the field of laser-scanning, confocal microscopy. Optical and Quantum Electronics 24, 851-859.
34. *Hird, S.N. & White, J.G. (1993) Cortical and Cytoplasmic Flow Polarity in early Caenorhabditis elegans. J.C.B. 121, 1343-1355.
35. Goldstein, R., Hird, S.N. & White, J.G. (1993) Cell polarity in Caenorhabditis elegans. Development Supplement 279-287.
36. *Podbilewicz, B. & White, J.G. (1994) Cell Fusions in developing epithelia in Caenorhabditis elegans. Dev. Biol. 161:408-423
37. *Newman, A.P., White, J.G. and Sternberg, PW. (1995) The C. elegans lin-12 gene mediates induction of ventral uterine specialization by the anchor cell. Development 121:263-271.
38. White, J.G. & Strome, S. (1996) Cleavage plane specification in C. elegans: how to divide the spoils. Cell 18: 195-198.
39. Wokosin, D.L. Contonze, V.E. White, Hird, S.N., Sepsenwol, S., Malcolm, G.P.A., Maker, G.T. and A.I. Ferguson (1996) Multiple-photon excitation imaging with an all solid-state laser Proc. SPIE 2678: no.83
40. Ferguson, A. and White, J.G. (1996) Solid-state lasers improve bio-imaging applications Photonics Spectra 30:100-103.
41. *Newman, A.P., White, J.G. and Sternberg, P. (1996) Morphogenesis of the C. elegans hermaphrodite uterus. Development - 122(11):3617-3626.
42. *Thomas, C., DeVries, P., Hardin, J. and White, J. (1996) Four-dimensional imaging: computer visualization of 3D movements in living specimens. Science 273: 603-607.
43. *Wokosin, D.L., Centonze, V.C., Crittenden, S. White, J. (1996).Three-photon excitation fluorescence imaging of biological specimens using an all-solid-state laser. Bioimaging 4: 208-214.
44. *Wokosin, D.L., Contonze, V., White, J.G., Armstrong, D., Robertson, G. and Ferguson, A.I. (1996) All-solid-state ultrafast lasers facilitate multiphoton excitation fluorescence imaging. IEEE Journal of Selected Topics in Quantum Electronics. 2(4):1051-1065.
45. White, J.G. (1997) Photons combine to visualize living tissue. Physics World 10(5):26-27.
46. Centonze, V.C. and White, J.G. (1997) Multiphoton excitation fluorescence microscopy. In “Cells a Laboratory Manual”. Eds. Golman, R., Leinward, L. and Spector, D.L. Cold Spring Harbor Press, Cold Spring Harbor, New York.
47. *Centonze, V.C. and White, J.G. (1998) Multiphoton excitation provides optical sections from deeper within scattering specimens than confocal imaging. J. Biophysics 75: 2015-2024.
48. *O’Connell, K.F., Leys, C.M. and White, J. (1998) Temperature sensitive cell division mutants of Caenorhabditis elegans. Genetics 149: 1303-1309.
49. Thomas, C. and White, J.G. (1998) 4D Imaging: The Exploration of Time and Space. Trends in Biotechnology -in press, April Issue.
50. Wokosin, D.L. and White, J.G. (1998) Multiphoton excitation fluorescence imaging applied to the study of embryo development. Proc. SPIE 3269: 86-92.
51. Wokosin, D.L. and White, J.G. (1998) Pulse-width considerations for multiple-photon excitation laser-scanning fluorescence imaging. Proc SPIE 3260a: 115-122.
52. Wokosin, D.L. and White, J.G. (1997) Optimization of the design of a multiphoton, excitation, laser-scanning fluorescence imaging system. Proc SPIE 2984: 24-29.
53. Mohler, W.A. and White, J.G. (1998) Stereo-4D reconstruction and animation from living fluorescent specimens. BioTechniques 24: 1006-1010.
54. Mohler, W.A. and White, J.G. (1998) Multiphoton laser scanning microscopy for four-dimensional analysis of Caenorhabditis elegans embryonic development. Optics Express 3: 325-331.
55. *Keating H.H. and White, J.G. (1998) Centrosome dynamics in early embryos of Caenorhabditis elegans. (1998) J. Cell Sci. 111: 3027-3033.
56. *Mohler, W.A., Simske, J.S., Williams-Masson, E.M., Hardin, J.D., and White J.G. (1998) Dynamics and ultrastructure of developmental cell fusions in the Caenorhabditis elegans hypodermis. Current Biology 8: 1087-1090.
57. *Skop, A.R. and White. J.G. (1998) The dynactin complex is required for cleavage plane specification in early Caenorhabditis elegans embryos Current Biology 8: 1110-1116.
58. *Sharma-Kishore, R., White, J.G., Southgate, E. and Podbilewicz, B. (1999) Formation of the vulva in Caenorhabditis elegans: a paradigm for organogenesis. Development 126: 691-699.
59. *Hockberger PE, Skimina TA, Centonze VE, Lavin C, Chu S, Dadras S, Reddy JK and White JG (1999) Activation of flavin-containing oxidases underlies light-induced production of H2O2 in mammalian cells. Proc Natl Acad Sci U S A. 96(11):6255-6260.
60. *Squirrell, J.M., Wokosin, D., White, J.G., and Bavister, B. (1999). Long-term multiphoton fluorescence imaging of mammalian embryos does not compromise viability. Nature Biotechnology 17: 763-767.
61. *O’Connell, K.F., Maxwell, K.N. and White, J.G. (2000) The spd-2 gene is required for polarization of the anterior/posterior axis and formation of the sperm asters in the Caenorhabditis elegans zygote. Dev. Biol. 222: 55-70.
62. *Nguyen, T.Q., Sawa, H., Okano, H. and White, J.G. (2000) The C. elegans septin genes, unc-59 and unc-61, are required for normal postembryonic cytokineses and morphogenesis but have no essential function in embryogenesis. J. Cell Sci. – 113:2825-3837.
63. White, J. (2000) Worm tales. Int. J. Dev. Biol. 44: 39-42.
64. Strome, S., Powers, J., Dunn, M., Reese, M., Malone, C., White, J. and Saxton, W. (2001) Spindle dynamics and the role of γ-tubulin in early C. elegans embryos. Mol. Biol. Cell 12: 1751-1764.
65. *O’Connell, K.F., Caron, C, Kopish, K.R., Hurd, D. H., Kemphues, K.J., Li, Y. and White, J.G. (2001) The C. elegans zyg-1 gene encodes a novel regulator of centrosome duplication with distinct maternal and paternal roles in the embryo. Cell 105:547-558.
66. *Skop, A.R., Bergmann, D., Mohler, W.A. and White, J.G. (2001) Completion of cytokinesis in C. elegans requires a brefeldin A-sensitive membrane accumulation at the cleavage furrow apex. Current Biol. 11:735-746.
67. White, J.G., Squirrell, J.M. and Eliceiri, K.W. (2001) Applying multiphoton imaging to the study of membrane dynamics in living cells. Traffic 2: 775-780.
68. *Siomos, MF, Badrinath, A, Pasierbek, P., Livingstone, D., White, J., Glotzer, M and Naysmyth, K. (2001) Separase is required for chromosome segregation during meiosis in Caenorhabditis elegans. Current Biology 11:1825-1835.
69. *Mohler, W.A., Shemer, G., del Campo, J.J., Volansi, C., Opoku-Serebuoh, E., Scranton, V., Assaf, N., White, J.G. and Podbilewicz, B. (2002) The type I membrane proteinEFF-1 is essential for developmental cell fusion. Dev Cell 2:355-362.
70. Finger F.P. and White J.G. (2002) Fusion and fission: membrane trafficking in cytokinesis. Cell 108:727-730.
71. Wang, W., Wyckoff, J.B., Frohlich, V.C., Oleynikov, Y., Huttelmaier, S., Zavadil, J., Cermak, L., Bottinger, E.P., Singer, R.H., White, J.G., Segall, J.E. and J.S. Condeelis. (2002) Single cell behavior in metastatic primary mammary tumors correlated with gene expression patterns revealed by molecular profiling. Cancer Res 62:6278-6288.
72. *Wokosin, D.L., Squirrell, J.M., Eliceiri, K.W. and White, J.G. (2003) An optical workstation with concurrent, independent multiphoton imaging and experimental laser microbeam capabilities. Rev Sci Instr 74:1-9.
73. *Badrinath, A.S. and J.G. White (2003) Contrasting patterns of mitochondrial redistribution in the early lineages of Caenorhabditis elegans and Acrobeloides sp. PS1146 Dev Biol 258:70-75.
74. *Eliceiri, K.W., Fan, C.H., Lyons, G.A. and J.G. White (2003) Analysis of histology specimens using lifetime multiphoton microscopy. Biomed Optics – 8:376-380.
75. * Finger, F.P. Kopish, K.R. and White, J.G. (2003) A role for septins in cellular and axonal migration in C. elegans Dev. Biol 261:220-234.
76. Amos, W.B. and J.G. White (2003) How the laser scanning confocal microscope entered biological research. Biol. Of the Cell 95:335-352.
77. White, J. and S. Bednarek (2003) Cytokinesis: GAGs form the walls that separate our parts. Current Biol. 13: R717-R718.
78. *Malone CJ, Misner L, Le Bot N, Tsai MC, Campbell JM, Ahringer J, White JG. (2003) The C. elegans hook protein, ZYG-12, mediates the essential attachment between the centrosome and nucleus. Cell. 2003 115:825-36.
79. *Rueden C., Eliceiri K.W. and J.G. White. (2004). VisBio: A Computational Tool for Visualization Multidimensional Biological Image Data. Traffic. Jun;5:1-7
80. *Stefansson, H.N., Eliceiri K.W., Thomas C.F., Ron A., DeVore R., Sharpley R., and J.G. White. Wavelet Compression of Three-Dimensional Time-lapse Biological Image Data. (2005) Microscopy and Microanalysis. 11:9-17.
81. *Bird, D.K., Eliceiri, K. W., Fan, C-H. and White, J. G., (2004) Simultaneous two-photon spectral and lifetime fluorescence microscopy. Applied Optics, 2004 43:5173-5182.
82. *Verbrugghe, K.J. and J.G. White (2004) SPD-1 is required for the formation of the spindle midzone but is not essential for the completion of cytokinesis in C. elegans embryos. Current Biology 14:1766 1760.
83. Poteryaev D., Squirrell J.M., Campbell J.M., White J.G., Spang A. (2005). Involvement of the actin cytoskeleton and homotypic membrane fusion in ER dynamics in Caenorhabditis elegans. Mol Biol Cell. 16:2139-2153.
84. Bird, D.K., Long, Y., Vrotsos, K.M., Eliceiri, K.W., Vaughn, E.M., Keely, J.K., White, J.G. and Ramanujam, N. (2005) Metabolic mapping of MCF10A human breast cells via multiphoton fluorescence lifetime imaging of the coenzyme NADH. Cancer Res 65:8766-8773.
85. *Squirrell, J.M., Eggers, Z.T., Luedke, N., Saari, B., Grimson, A., Lyons, G.E., Anderson, P. and White, J.G. (2006) CAR-1, a protein that localizes with the mRNA decapping component DCAP-1, is required for cytokinesis and ER organization in Caenorhabditis elegans. Mol Biol Cell 17:336-344.
86. Provenzano, P.P., Eliceiri, K.W., Campbell, J.M., Inman, D.R., White, J.G., Keely, P.J. (2006) Collagen reorganization at the tumor-stromal interface facilitates local invasion. BMC Med. 4:38.
87. Bird, D.K., Eliceiri, K.W. and White, J.G. (2006) Optimized temporal response in multichannel two-photon fluorescence microscopy using a photonics crystal fibre. J. Microscopy 224:249-255.
88. *Verbrugghe, K.J. and White, J.G. (2007) Cortical centralspindlin and G_ have parallel roles in furrow initiation in early C. elegans embryos. J.C.S. 120: 1772-1778.
89. Lu, F.-M., Eliceiri, K.W., Stewart, J. and White, J.G. (2007) WormClassroom.org: An Inquiry-rich Educational Web Portal for Research Resources of Caenorhabditis elegans CBE Life Science Education 6:98-108.
90. Lu, F., Eliceiri, K. W., & White, J. G. (2007). BioClips of symmetric and asymmetric cell division. Biol Cell, 99(5): 289-295.
91. *Batchelder E.L., Thomas-Virnig C.L., Hardin J.D. & White J.G. (2007) Cytokinesis is not controlled by calmodulin or myosin light chain kinase in the Caenorhabditis elegans early embryo. FEBS Lett. 581(22): 4337-4341.
92. *Bembenek, J.N., Richie, C.T, Squirrell, J.M., Campbell, J.M., Kevin W. Eliceiri, K.W., Poteryaev, D., Spang, A., Golden, A and White, J.G. (2007) Cortical granule exocytosis in C. elegans is regulated by cell cycle components including separase. Development 134: 3837-3848.
93. Skala, M.C., Riching, K.M., Gendron-Fitzpatrick, A., Erickhoff, J., Eliceiri, K.W., White, J.G. and Ramanujam N. (2007)In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia. PNAS 104(49): 19494-19499.
94. *Dinkelmann, M.V., Zhang, H, Skop, A.R. and White, J.G. (2007) SPD-3 is required for spindle alignment in Caenorhabditis elegans embryos and localizes to mitochondria. Genetics 177(3): 1609-1620.
95. *Zhang, H, Skop, A.R. and White, J.G. (2008) Src and Wnt signaling regulate dynactin accumulation to the P2-EMS cell border in C. elegans embryos. J.C.S. 121: 155-161.
96. Provenzaono, P.P., Inman, D.R., Eliceiri, K.W., Knittel, J.G., Yan, L., Rueden, C.T., White, J.G. and Keely, P.J. (2008) Collagen density promotes mammary tumor initiation and progression. BMC Medicine 6:11
97. * Zhang, H., Squirrell, J.M. and White, J.G. (2008) RAB-11 Permissively Regulates Spindle Alignment by Modulating Metaphase Microtubule Dynamics in Caenorhabditis elegans Early Embryos. Mol. Biol. Cell 19(6):2553-2465.