JOVE protocols

Behaviour

Temperature Sensation

• Local and Global Methods of Assessing Thermal Nociception in Drosophila Larvae

• Design and Analysis of Temperature Preference Behavior and its Circadian Rhythm in Drosophila

Locomotion, Circadian Rhythm and Sleep Behavior

• Methods to Assay Drosophila Behavior

• Assaying Locomotor Activity to Study Circadian Rhythms and Sleep Parameters in Drosophila

• High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster

• Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

• Quantitative Measurement of the Immune Response and Sleep in Drosophila

Learning and Memory

• Assaying Locomotor, Learning, and Memory Deficits in Drosophila Models of Neurodegeneration

• Operant Learning of Drosophila at the Torque Meter

• Appetitive Associative Olfactory Learning in Drosophila Larvae

Aggression

• Studying Aggression in Drosophila (fruit flies)

Taste and Feeding

• Functional Analysis of the Larval Feeding Circuit in Drosophila

• Proboscis Extension Response (PER) Assay in Drosophila

• A Single-fly Assay for Foraging Behavior in Drosophila

• Simultaneous Recording of Calcium Signals from Identified Neurons and Feeding Behavior of Drosophila melanogaster

Odor-driven Behavior

• Visually Mediated Odor Tracking During Flight in Drosophila

• A Magnetic Tether System to Investigate Visual and Olfactory Mediated Flight Control in Drosophila

• High-resolution Measurement of Odor-Driven Behavior in Drosophila Larvae

Ethanol Sensitivity

• A Simple Way to Measure Ethanol Sensitivity in Flies

Lifespan, Exercise and Neurodegenerative Effects on Behavior

• Assessing Neurodegenerative Phenotypes in Drosophila Dopaminergic Neurons by Climbing Assays and Whole Brain Immunostaining

• Measurement of Lifespan in Drosophila melanogaster

• Endurance Training Protocol and Longitudinal Performance Assays for Drosophila melanogaster

Organ Function

• Visualizing the Beating Heart in Drosophila

• Experimental Manipulation of Body Size to Estimate Morphological Scaling Relationships in Drosophila

• Assessing Differences in Sperm Competitive Ability in Drosophila

• Isolation of Drosophila melanogaster Testes

Controlling & Measuring Neuronal Activity

• Optogenetic Perturbation of Neural Activity with Laser Illumination in Semi-intact Drosophila Larvae in Motion

• Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster

• Preparation of Drosophila Central Neurons for in situ Patch Clamping

• Whole Cell Recordings from Brain of Adult Drosophila

• Insulin Injection and Hemolymph Extraction to Measure Insulin Sensitivity in Adult Drosophila melanogaster

• Single Sensillum Recordings in the Insects Drosophila melanogaster and Anopheles gambiae

• Loading Drosophila Nerve Terminals with Calcium Indicators

Biochemistry and Molecular Biology

• Identifying Protein-protein Interaction in Drosophila Adult Heads by Tandem Affinity Purification (TAP)

• Recombineering Homologous Recombination Constructs in Drosophila

• RNAi Interference by dsRNA Injection into Drosophila Embryos

• Purification of Transcripts and Metabolites from Drosophila Heads

• Identification of Olfactory Volatiles using Gas Chromatography-Multi-unit Recordings (GCMR) in the Insect Antennal Lobe

Imaging, Staining and Cell Biology

Adult Visual System

• Single Drosophila Ommatidium Dissection and Imaging

• Preparation of Adult Drosophila Eyes for Thin Sectioning and Microscopic Analysis

• Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging

Larval and Pupal Nervous System

• Drosophila Larval NMJ Immunohistochemistry

• Dissection of Larval CNS in Drosophila Melanogaster

• Dissection and Immunohistochemistry of Larval, Pupal and Adult Drosophila Retinas

• Visualization of Proprioceptors in Drosophila Larvae and Pupae

• Drosophila Pupal Abdomen Immunohistochemistry

• In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons

• In vivo Imaging of Intact Drosophila Larvae at Sub-cellular Resolution

Brain and CNS

• Ex vivo Culturing of Whole, Developing Drosophila Brains

• Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

• An Injury Paradigm to Investigate Central Nervous System Repair in Drosophila

• Neurocircuit Assays for Seizures in Epilepsy Mutants of Drosophila

• Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS

Cell Isolation from Flies

• Dissection of Oenocytes from Adult Drosophila melanogaster

• Laser Capture Microdissection of Drosophila Peripheral Neurons

• Isolation and Purification of Drosophila Peripheral Neurons by Magnetic Bead Sorting

Cell Isolation from Flies

• Dissection of Oenocytes from Adult Drosophila melanogaster

• Laser Capture Microdissection of Drosophila Peripheral Neurons

• Isolation and Purification of Drosophila Peripheral Neurons by Magnetic Bead Sorting

Cell Culture

• Preparation of Drosophila S2 cells for Light Microscopy

• Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae

This list is curated by https://labs.mcdb.ucsb.edu/montell/craig/protocols/jove-protocols