Publications

Spiegel Lab Publications († - undergraduate author):

Belofski G, Ahn H, Zapata M, Wilcox D, Salomon CE, Spiegel PC Jr. (2023) Antimicrobial Isoflavans and Other Metabolites of Dalea jamesii (Fabaceae). Planta Medica Online ahead of print.

*Peters SC, Childers KC, *Mitchell CE, †Avery NG, †Reese SS Jr, †Mitchell C, *Wo SW, *Swanson CD, †Brison CM, Spiegel PC Jr. (2022). Stable binding to phosphatidylserine-containing membranes requires conserved arginine residues in tandem C domains of blood coagulation factor VIII. Frontiers in Molecular Biosciences 9:1040106.

McPherson V, Wolhart H, Engels L, Sullivan K, Coria A, Bicchieri T, Torrey B, Spiegel PC Jr, Danforth S, Belofsky G, Dondji B. (2022). Assessment of the anthelmintic activity and toxicity of metabolites of Dalea pogonathera (Fabaceae). Phytochem Letters 51(2022): 75-81.

Spiegel PC Jr. (2022). Shining a Light on Thrombin Generation. Blood 139(24): 3451-3453.

Childers KC, *Peters SC, Lollar P, Spencer HT, Doering CB, Spiegel Jr PC. (2022). SAXS Analysis of Intrinsic Tenase Complex Bound to Lipid Nanodisc Highlights Intermolecular contacts between factors VIIIa/IXa. Blood Advances 6(11): 3240-3254.

Spiegel PC Jr. (2021). VWF Self-Association Requires Tensile Force. Blood 138(23): 2309-2310.

Krishnaswamy S, Ageno W, Arabi Y, Barbui T, Cannegieter S, Carrier M, Cleuren AC, Collins P, Panicot-Dubois L, Freedman JE, Freson K, Hogg P, James AH, Kretz CA, Lavin M, Leebeek FWG, Li W, Maas C, Machlus K, Makris M, Martinelli I, Medved L, Neerman-Arbez M, O'Donnell JS, O'Sullivan J, Rajpurkar M, Schroeder V, Spiegel PC Jr, Stanworth SJ, Green L, Undas A. (2021). Illustrated State-of-the-Art Capsules of the ISTH 2020 Congress. Res Pract Thromb Haemost 5(5): e12532.

†Ronayne EK, *Peters SC, *Gish JS, †Wilson C, Spencer HT, Doering CB, Lollar P, Spiegel Jr PC, Childers KC. (2021). Structure of Blood Coagulation Factor VIII in Complex With an Anti-C2 Domain Non-Classical, Pathogenic Antibody Inhibitor. Frontiers in Immunology 12: 2260.

*Gish JS, †Jarvis L, Childers KC, †Peters SC, †Garrels CS, *Smith IW, Spencer HT, Doering CB, Lollar P, Spiegel PC Jr. (2021). Structure of Blood Coagulation Factor VIII in Complex with an Anti-C1 Domain Pathogenic Antibody Inhibitor. Blood 137(21): 2981-2986. Cover Article

*Smith IW, †d'Aquino AE, Coyle CW, Fedanov A, Parker ET, Denning G, Spencer HT, Lollar P, Doering CB, Spiegel PC Jr. (2020). The 3.2 Å structure of a bioengineered variant of blood coagulation factor VIII indicates two conformations of the C2 domain. Journal of Thrombosis and Haemostasis, 18(1): 57-69.

*Vincent CT, †Long ET, †Jones HC, Young JC, Spiegel PC, O'Neil GW. (2019) Suzuki Coupling-based Synthesis of VATPase Inhibitor Archazolid Natural Product Derived Fragments. RSC Advances 9(55): 32210-32218.

*Carlson MA, †Haddad BG, *Weis AJ, †Blackwood CS, †Shelton CD, †Wuerth ME, *Walter JD, Spiegel PC. (2017). Ribosomal Protein L7/L12 is Required for GTPase Translation Factors EF-G, RF3 and IF2 to Bind in their GTP State to 70S Ribosomes. FEBS J 284(11):1631-1643.

O’Neil GW, †Craig AM, *Williams JR, Young JC, Spiegel PC. (2017) Synthesis of the C1-C23 Fragment of the Archazolids and Evidence for V-ATPase but not COX Inhibitory Activity. Synlett 28(09): 1101-1105.

*Wuerth ME, †Cragerud, RK, Spiegel PC. (2015). X-ray Crystal Structure of the Human Factor VIII C2 Domain in Complex with the Classical 3E6 Inhibitory Antibody. Scientific Reports 5:17216

†Brison CM, †Mullen SM, *Wuerth ME, †Podolsky K, †Cook M, †Herman JA, *Walter JD, Meeks SL, Spiegel PC. (2015). The 1.7 Å X-ray Crystal Structure of the Porcine Factor VIII C2 Domain and Binding Analysis to Anti-Human C2 Domain Antibodies and Phospholipid Surfaces. PLoS One 10(3): e0122447.

Sevy AM, Healey JF, Deng W, Spiegel PC, Meeks SL, Li R. (2013). Epitope mapping of inhibitory antibodies targeting the C2 domain of coagulation factor VIII by hydrogen-deuterium exchange mass spectrometry. J Thromb Haemost 11(12): 2128-36.

Walter JD, Werther RA, †Brison CM, †Cragerud RK, Healey JF, Meeks SL, Lollar P, Spiegel PC Jr. (2013). Structure of the factor VIII C2 domain in a ternary complex with 2 inhibitor antibodies reveals classical and nonclassical epitopes. Blood 122(26): 4270-8.

Walter JD, Werther RA, †Polozova MS, Healey JF, Meeks SL, Lollar P, Spiegel PC. (2013). Characterization and Solution Structure of the Factor VIII C2 Domain in a Ternary Complex with Classical and Non-Classical Antibody Inhibitors.  Journal of Biological Chemistry 288(14):9905-14. 

Walter JD, †Hunter M, †Cobb M, †Traeger G, Spiegel PC. (2012). Thiostrepton Inhibits Stable 70S Ribosome Binding and Ribosome-Dependent GTPase Activation of Elongation Factor G and Elongation Factor 4.  Nucleic Acids Research 40(1): 360-70. 

Karanicolas J, Corn JE, Chen I, Joachimiak LA, Dym O, Chung S, Albeck S, Unger T, Hu W, Liu G, †Delbecq S, Montelione G, Spiegel C, Liu DR, Baker D. (2011). A De Novo Protein Binding Pair by Computational Design and Directed Evolution.  Molecular Cell. 42(2): 250-60. 

Walter JD, †Littlefield P, †Delbecq S, Prody G, Spiegel PC. (2010). Expression, Purification, and Analysis of Unknown Translation Factors from Escherichia coli:  A Synthesis Approach. Biochemistry and Molecular Biology Education. 38(1): 17-22. 

Previous Publications:

Shen BW*, Spiegel PC*, et. al., Stoddard, BL.  (2008). Tertiary Structure and Domain Organization of Coagulation Factor VIII.  Blood. 111(3): 1240-7.  * Co-first author.

Spiegel PC, Ermolenko DN, Noller HF. (2007).  Elongation Factor G Stabilizes the Hybrid-State Conformation of the 70S Ribosome.  RNA. 13(9): 1473-82.

Ermolenko DN, Majumdar ZK, Hickerson RP, Spiegel PC, Clegg RM, Noller HF. (2007).  Observation of Intersubunit Movement of the Ribosome in Solution Using FRET.  Journal of Molecular Biology.  370(3): 530-40.

Ermolenko DN*, Spiegel PC*, Majumdar ZK, Hickerson RP, Clegg RM, Noller HF.  (2007).  The Antibiotic Viomycin Traps the Ribosome in an Intermediate State of Translocation.  Nature Structural and Molecular Biology.  14(6): 493-7. *Co-first author.

Spiegel PC, Chevalier B, Sussman D, Turmel M, Lemieux C, Stoddard BL.  (2006). The Structure of I-CeuI Homing Endonuclease: Evolving Asymmetric DNA Recognition from a Symmetric Protein Scaffold.  Structure. 14(5): 869-880.

Spiegel PC, Murphy P, Stoddard BL.  (2004).  Surface-exposed hemophilic mutations across the factor VIII C2 domain have variable effects on stability and binding activities.  Journal of Biological Chemistry.  279(51): 53691-8.  Cover Article

Spiegel PC, Kaiser SM, Simon JA, Stoddard BL.  (2004).  Disruption of protein-membrane binding and identification of small molecule inhibitors of coagulation factor VIII.  Chemistry and Biology.  11(10): 1413-22.

Bolduc JM, Spiegel PC, Chatterjee P, Caprara MG, Waring RB Stoddard BL.  (2003).  The structure and DNA recognition of a bifunctional homing endonuclease and group I intron splicing factor.  Genes and Development.  17(23): 2875-88.  Cover Article

Spiegel PC, Jacquemin M, Saint-Remy JM, Stoddard BL, Pratt KP.  (2001).  Structure of a factor VIII C2 domain-immunoglobulin G4kappa Fab complex:  identification of an inhibitory antibody epitope on the surface of factor VIII.  Blood.  98(1): 13-9. Plenary Article

Book Chapters and Reviews:

Childers KC, *Peters SC, Spiegel PC Jr. (2022). Structural insights into blood coagulation factor VIII: Procoagulant complexes, membrane binding, and antibody inhibition. J Thromb Haemostasis 20(9): 1957-1970. Cover Article, Review

Noller HF, Ermolenko DN, Korostelev A, Laurberg M, Zhu J, Asahara H, Lancaster L, Horan L, Hirschi A, Donohue JP, Trakhanov S, Spiegel C, Hickerson R, Cornish P, Ha T. (2011). Studies on the Mechanisms of Translocation and Termination. Ribosomes.  Ch 28. ISBN 978-3-7091-0214-5.

Spiegel PC and Stoddard BL.  (2002).  Optimization of factor VIII replacement therapy: can structural studies help in evading antibody inhibitors?  British Journal of Haematology.  119(2): 310-22.  Review