A cheap/rapid technique for fabrication of μPADs is presented for point of care analysis. Hydrophobic channels were formed across the whole width of paper. The process yielded uniform 563±5 μm hydrophilic microchannels, without the use of the clean room, UV lamp, or organic solvents and was completed in a single step without the need for a hotplate. Our method is advantageous in the cost and ease of fabrication (even at home), process time (less than one minute), the feasibility of mass-fabrication, multiplexing, readout and environmental considerations. Performance of the resulting μPAD was assessed on a multiplexed Uric acid and nitride assay, bearing 95% of confidence level in the readout against standardized tests. This combinatorial sensor demonstrates a good linear range (up to 500 mg/dL), low detection limit (49 mg/dL) and real-time analytic capabilities for portable and information technology-based diagnostic and health monitoring.