The LEE Single Molecule Biophysics LAB

The overall goal of our research team is to develop a “molecular mechanistic movie” for the operation of various molecular machines in biological system (including CRISPR-Cas DNA endonucleases, DNA topoisomerases, membrane fusion proteins and peptide synthetases). To achieve this goal, we use cutting-edge single-molecule imaging and manipulation techniques including single-molecule FRET and optical tweezers. As molecular systems of interest become more complex, however, conventional single-molecule technique is insufficient to investigate the complex bio-molecular dynamics, and there is an ever increasing demand for more advanced techniques. Hence, we have been also focused on the development of new single-molecule techniques including single-molecule multi-color FRET, optical tweezers combined with multi-color FRET, and single molecule probes for short distance sensitivity. And also, we have a strong interest to develop single-cell imaging and manipulation techniques based on biophotonics.


  • Y. Jang*, H. Son*, S.W. Lee, W. Hwang, S.R. Jung, J.A.W. Byl, N. Osheroff#, Sanghwa Lee# (2019) “Selection of DNA cleavage sites by topoisomerase II results from enzyme-induced flexibility of DNA” Cell Chemical Biology, 26, 502 (*equal contribution, #co-corresponding authors)
  • Y. Jeon*, Y.H. Choi*, Y. Jang*, J. Yu, J. Goo, G. Lee, Y.K. Jeong, S.H. Lee, I.-S. Kim, J.-S. Kim, C. Jeong#, Sanghwa Lee#, S. Bae# (2018) “Direct observation of DNA target searching and cleavage by CRISPR-Cas12a” Nature Communications, 9, 2777 (*equal contribution, #co-corresponding authors)