Study systems

Genetic and morphological variation in Syngnathus pipefishes

Students in the lab are developing and optimizing molecular markers to study population structure and genetic diversity in California's Syngnathus pipefishes. Graduate student Anabell Espinosa currently explores head shape variation and has constructed and annotated the mitochondrial genomes for S. leptorhychus, S. excilis, S. californiensis, S. euchrous, and S. auliscus.

Genetic structure of Greenland Sharks (Somniosus microcephalus)

Relatively little is known of the biology of Sleeper sharks. Partnering with researchers in Iceland, Norway, the Canadian Maritimes, and the USA, we are discovering a more complex evolutionary history among these long-lived elasmobranchs than previously thought. We have developed markers to characterize population structure from the Canadian Arctic and beyond (Swintek and Walter 2021 - BMC Research Notes). Previous work demonstrates the occurrence of Pacific Sleeper shark (Somniosus pacificus) genetic signatures among juvenile and adult Greenland sharks sampled in the Canadian Arctic (Hussey et al. 2014 - Polar Biology, Walter et al. 2017 - Ecology & Evolution), and further reports the admixed individuals well into the temperate and subtropical waters of the Atlantic. 

Transcriptomics of Southern California’s Native and Non-native Oysters

Olympia oysters (Ostrea lurida) are a historical foundation species in coastal California with a considerable role in maintaining ecosystem health and function in bays and estuaries. We are examining the comparative transcriptomics of Southern California’s native O. lurida and non-native Pacific oyster (Crassostrea gigas) in response to predicted climate change stressors, including fluctuating temperatures and salinities.

Upper Newport Bay, California. Photo credit: Stacey McIntyre

Genetic admixture in Manta Rays

Mantas are large mobile marine elasmobranchs exhibiting a variety of genetic, morphological, and ecological characteristics.  Recent taxonomic revision has reclassified Mantas into two species on the basis of morphology, although we are finding new and exciting data blurring these boundaries (Walter et al. 2014 - Marine Biodiversity; Hinojosa-Alvarez et al. 2016 - PeerJ).

 Manta alfredi, Dunganab Bay, Sudan - 2012. Photo credit: C. Braun

Molecular Ecology of Eastern Sand Darter

We are currently focusing on geographic and temporal population genetic structure in Eastern Sand Darter (Ammocrypta pellucida) and Greenside Darter (Etheostoma blennioides). Our recent papers in Ecology and Evolution (Ginson et al. 2015) and Journal of Fish Biology (Walter et al. 2021) show riverine isolation in A. pellucida, but a lack of differentiation among individuals collected from sites within rivers. These findings suggest that for a given river, A. pellucida disperses throughout the system, likely as a result of the instability of its sandy depositional habitat.   

O'opu - Hawaiian stream fishes

The Hawaiian archipelago is home to a number of amphidromous fish species, all of which exhibit a seemingly obligate marine larval phase. Their unique life history highlights vulnerabilities to changing global environmental conditions (Walter et al. 2012 - ESR). Four endemic species (Eleotris sandwicensis, Sicyopterus stimpsoni, Stenogobius hawaiiensis, Lentipes concolor) and one indigenous species (Awaous guamensis) are the focus of a broad phylogeographic study of genetic connectivity among watersheds and islands. Through a collaboration with researchers at Tulane University, NC State, and the University of Wisconsin-Madison, we have uncovered the existence of an endemic Awaous lineage among the Hawaiian islands (Lindstrom et al. 2012 - Copeia). 

O'opu nakea - Awaous stamineus - Pelekunu, Moloka'i - 2011

O'opu alamo'o - Lentipes concolor (male) - Hanakapi'ai, Kaua'i 2011

O'opu nopili - Sicyopterus stimpsoni, Pelekunu, Moloka'i - 2009

Myself, Evan Childress, Derek Hogan, and Roderick Gagne in Kalawao, 

near Kalaupapa, Moloka'i - April 2011 - Photo credit: Eric Brown

5 min video of Awaous stamineus and Sicyopterus stimpsoni in Pelekunu, Moloka'i, 2009.

Sailfin silversides

Sailfin silversides (Telmatherinidae) are a family of freshwater and brackish fishes found on Sulawesi and Papua, Indonesia. The Sulawesi species represent one of the best examples of sympatrically adaptively-radiating tropical freshwater fishes outside of the African cichlid species flocks. Telmatherina antoniae is endemic to Lake Matano in southern Sulawesi. Our work with T. antoniae shows remarkable patterns of genetic differentiation in lieu of their extensive dispersive capacity and potential for high gene flow throughout their distribution (Walter et al. 2009 - JEB). Distinct blue, yellow and mixed colour morphs co-occur in this species, and while some assortative courtship has been observed, there does not appear to be any underlying differentiation among colour morphs (Walter et al. 2009 - JFB). More recently we've uncovered differential patterns of lake-specific genetic divergence coupled with inter-lake dispersal (Walter et al. 2011 - Conserv Gen) in two species of Paratherina, a genus endemic to the southern Malili Lakes of Sulawesi. 

Telmatherina antoniae, an endemic freshwater fish from Lake Matano, Sulawesi, Indonesia - 2006.

Male blue morphs of T. antoniae displaying their iridescent second dorsal and anal fins.

Coral Reef Ecology

Diving and working on coral reefs is a passion I've held since my undergraduate years. As coral reefs are among the most speciose of aquatic environments, complex interactions exist among members of the coral reef community. Many fishes are associated with corals, algae and other reef-dwelling invertebrates at  some point in their lives. My MS with Jim Haynes (SUNY Brockport) examined comparative community structure among fishes and coral species on the shallow-water patch reefs of San Salvador, Bahamas (Walter & Haynes 2006 - Bull Mar Sci). I continue to work on a number of studies examining regional and local connectivity in coral reef fish species along the MesoAmerican Barrier Reef System in the Caribbean (Salas et al. 2010 - Mar Biol; Hogan et al. 2010 - Mol Ecol Notes).  

Bicolor damselfish (Stegastes partitus), Turneffe Atoll, Belize.

Coney (Cephalopholis fulva), with French grunts (Haemulon flavolineatum), Snapshot reef, San Salvador, Bahamas.

All photos by RP Walter except where otherwise indicated.