2009-10-15 - Introduction

Welcome and Introduction

• Described main goals:
  • Discuss ways that high-throughput sequencing can be used
  • Describe techniques that work and don't work
  • Guide development of sequencing pipeline
• Sequencing center has one machine now, hoping to get a second machine soon. More machines will help to reduce the backlog of samples.
• All data from the center must be shared upon publication.

Data Storage Requirements

• Center will keep data safe
• Keeping it safe ends at the reads, subsequently generated data from analysis will be the researchers responsibility, though the center will assist.
• Storing images is not generally useful, though there is the possibility of getting more data using improved software. Improvements in chemistry and hardware are likely to be even greater.

Member Introductions

• Each person introduced themselves and talked a bit about how they are using high-throughput sequencing.

Sample Information Collection

• Discussion of what pieces of data are necessary to collect during sample submission.
• Additional useful items to track include: Flowcell ID, Multiplex/Barcode Primers
• Need to consider a way to incorporate externally generated sequence data in to the Princeton pipelines for tracking and analysis.

Wrap-up

• Meetings will be held every two weeks. Schedule is posted on the wiki.