Date: Nov. 9, 2018
Place: Tokyo Waterfront, National Institute of Advanced Industrial Science and Technology
Program:
[Lectures]
1. Production of useful biochemicals by genetically-engineered fission yeast
Kaoru Takegawa (Department of Bioscience & Biotechnology, Faculty of Agriculture, Kyushu University)
2. Genome editing and new mating-type switching system in methylotrophic yeast
Yoshinobu Kaneko (Department of Biotechnology, Graduate School of Engineering, Osaka University)
Since Ogata et al. at Kyoto University isolated a yeast that can use methanol as a carbon source from nature in 1969, methylotrophic yeasts have been used as an important and useful microorganism in the industrial and academic fields, such as single-cell protein or heterologous protein production, biological studies on methanol and nitrate assimilation and peroxisome formation. Ogataea polymorpha (syn. Hansenula polymorpha) gains an advantage of industrial use over other methylotrophic yeasts, because classical genetic and molecular genetic techniques are applicable and the maximum growth temperature is 45-50 ºC. Here, I would like to talk about application of CRISPR/Cas9 genome editing and a new mating-type switching system associated with chromosomal inversion in O. polymorpha.
[MINCY salon]