Bioinstrumentation syllabus
Unit I Separation techniques, chromatography and centrifugation: General principle of separation, paper chromatography, thin layer, affinity, gel permeation, ion exchange, GLC, HPTLC, preparative and analytical centrifugations and their application
Unit II Electrophoresis and Spectroscopy: Basic principles of electrophoresis, Factor affecting electrophoretic mobility, native and denaturing PAGE, isoelectric focussing, 2DE, Pulse field gel electrophoresis. Spectroscopy: Theory and applications of Ultra violet and visible spectroscopy, IR, Nuclear magnetic resonance, Mass and applications.
Practical based on theory
1. Centrifugation
a. Isolation of cell organelles like cell membrane, mitochondria, ribosomes etc.
b. Determination of molecular weight of protein by centrifugation
2. Chromatography
a. To separate and identify the amino acids in a mixture by thin layer chromatography.
b. Separation of plant pigments by paper/TLC
c. Purification of proteins by Column Chromatography
3. Electrophoresis
a. Separation of soy bean proteins by PAGE
4. Spectroscopy
a. Validation of Lambert-Beer’s law (Photometer)
b. Estimation of DNA by DPA method (UV spectrophotometer)
c. Estimation of reducing sugars by DNSA method (VIS-Spectro.)
Recommended Books
1. Practical Biochemistry Paperback – 2016
2. An Introduction to Practical Biochemistry Paperback – 1 Jul 2017
3. Practical Biochemistry Paperback – Import, 31 Jan 2013
by R. C. Gupt
4. Practical Biochemistry: A Student Companion Paperback – Import, 10 Jul 2015
by Tiwari Anand
5. Laboratory Manual For Practical Biochemistry Paperback – 2013
6. A Text Book of Practical Biochemistry: 1 Paperback – Large Print, 1 Apr 2006