Bioinstrumentation
BTT 102/ BOT 102/ ZOT 102/MBT 102 (Common paper)
Unit I: Separation Techniques, Chromatography and Centrifugation
General principles and basis of separation, classification, separation, mechanism, paper chromatography, thin layer, affinity, gel permeation, ion exchange, GLC, HPTLC, preparative and analytical centrifugations and their applications
Unit II Electrophoretic TechniquesPrinciple andApplications
Basic principles of electrophoresis, Factors affecting electrophoresis, elephoretic mobility, paper and gel electrophoresis, native and denaturing PAGE, isoelectric focussing, 2D electrophoresis, pulse field gel electrophoresis,
Unit III Spectral Techniques
Theory and applications of: Ultra violet and visible spectroscopy, IR, Nuclear magnetic resonance, AAS, Mass and Raman spectroscopy, Fluorescence spectroscopy and X-ray spectroscopy and applications. Mass spectral detector, MS-MS and MALDI-SELDI-Chemical ionization, ESI techniques for proteins, Peptide mapping and sequence determination by MS-MS (TMS)
Unit IV Radiation and non-radiation techniques
Tracer technology, radioisotopes in biotechnology, Geiger-Muller counters, Metabolic tracer techniques, non-radioactive labels, labeling and detection methods using fluorescent molecules.
Practical based on theory
1. Centrifugation
a. Separation of blood cells
b. Determination of molecular weight of protein by centrifugation
c. Isolation of cell organelles like cell membrane, mitochondria, ribosomes etc.
2. Chromatography
a. Separation of amino acids by paper chromatography
b. Separation of sugars by TLC
c. Separation of plant pigments by paper/TLC
d. Purification of proteins by Column / ion exchange / Molecular sieve chromatography
3. Electrophoresis
a. Separation of soy bean proteins by PAGE
b. Determination of molecular weight by SDS PAGE
c. Isoelectric focussing
d. Separation of peptides by 2DE
e. Identification of amino acids by peptide mass fingerprinting
4. Spectroscopy
a. Validation of Lambert-Beer’s Law
b. Estimation of DNA by DPA method
c. Estimation of reducing sugars by DNSA method
References:
Bioanalytical Chemistry, By A. Manz, N. Pamme and D. Iossifidis World Scientific Publishing Company
Understanding Bioanalytical Chemistry Principles and applications Victor A. Gault and Neville H. McClenaghan School of Biomedical Sciences,University of Ulster, Northern Ireland, UK A John Wiley & Sons, Ltd., Publication
BIOANALYTICAL CHEMISTRY, Susan R. Mikkelsen and Eduardo Corton, Published by John Wiley & Sons, Inc., Hoboken, New Jersey.
BIOINORGANIC CHEMISTRY A Short Course Second Edition ROSETTE M. ROAT-MALONE, Chemistry Department Washington College Chestertown, MD Wiley-Interscience, A John Wiley & Sons, Inc., Publication
Principles and Techniques of Biochemistry and Molecular Biology Seventh edition Edited by Keith Wilson and John Walker Cambridge University Press, The Edinburgh Building, Cambridge CB2 8RU, UK
Fundamentals of Bioanalytical Techniques and Instrumentation by Sabaria.K. Srivastava Ghosal Publisher: PHI (2010)
Bioanalytical Techniques by Abhilasha Shourie Publisher: The Energy and Resources Institute (30 September 2015)
Bioanalytical Techniques by Sekhar Talluri Publisher: I K International Publishing House (15 March 2012)