Novel application of Single Molecule Localization Microscopy SMLM), such as dSTORM, in plant science finally allows the quantitative in situ chemical analysis of the cell wall nanostructure.
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© K.T. Haas. 3D dSTORM nanoscopy on developping cell plate.
Blue - Callose, Orange - Cellulose.
© K.T. Haas. The 3D 3-color dSTORM data scatterplot of the developping cell plate. Green- Callose, violet- crystalline cellulose, orange - mannan.
To mearn more:
Multicolor 3D-dSTORM reveals native-state ultrastructure of polysaccharides' network during plant cell wall assembly. iScience 2020, A. Peaucelle, R. Wightman & K. T. Haas, https://doi.org/10.1016/j.isci.2020.101862
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