The plant cell wall has to withstand very high hydrostatic pressure inside the cell (the turgor pressure). It is also highly dynamic and adaptable, permitting controlled cell and tissue growth. Novel chemical imaging tools as dSTORM nanoscopy now make it finally possible to resolve the actual structure of cell wall polymers and its changes during the growth, opening up a new directions of research, yet unforeseen. Multiplexed imaging with light-sensitive actuators (optogenetics) and multiparametric FRET biosensing allows the perturbations and observation of growth components at short timescales corresponding to initial cell wall response.