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July/August 2019 - Development of robust detection method at Foundation Plant Services (UC Davis)
Grapevine variety Ljutun in which grapevine bandavirus 1 was discovered for the first time. In the same plant grapevine virus G was confirmed, second finding after first report from New Zeland by Blouin and collaborators (Link)
September 2019 - March 2020 - testing the precise and robust diagnostic methods using conventional and real-time PCR at the Department of Plant Pathology (University of Zagreb).
April 2020 - transmission trials using mealybugs - crawlers of vine mealybug (Planococcus ficus) used as potential vector (left), Chenopodium murale plants used as potential hosts (center) and vines infected with GBV-1 and GVG as a source of viruses (right)
May 2020 - scheme of mechanical inoculation - as a potential hosts of GVG and GBV-1 several herbaceous/laboratory test plants, weeds commonly found in Croatian vineyards and vines grown from seeds were used
July/August 2020 - distribution survey - collecting the samples from commercial vineyards located in Croatian coastal region (islands Vis, Hvar, Mala Rava, Krk; region of Kaštela, Istrian peninsula (Poreč) and Zadar.
October 2020 - collecting the seeds from infected grapevine for transmission trials
February 2021 - grapevine cuttings from USA - second import of virus-free grapevine cuttings from Foundation Plant Services (UC Davis) and their rooting for transmission trials
March/June 2021 - transmission trials
Grafting
Transmission using vine mealybug (Pseudococcus ficus)
Mechanical inoculation
Seed transmission