VarGibbs implements a new optimization method which calculates nearest-neighbour entropies and enthalpies from melting temperatures. Parameters are available for DNA, RNA, and DNA-RNA hybrids. For DNA and RNA there are specific parameters at various salt concentrations.
Article in Bioinformatics: Optimization method for obtaining nearest-neighbour DNA entropies and enthalpies directly from melting temperatures (November 2014)
Read the documentation to see what VarGibbs can do for you.
News: version 4.1 is available with parameters for LNA modified DNA (May 2023) and DNA mismatches (October 2023)